Study on the development of simultaneous-rapid screening methods for residues pesticides in livestock products

수입 축산식품에서의 잔류농약 동시분석법 개발에 관한 연구

  • Kim, Jong-shu (College of Veterinary Medicine, Gyeonsang National University(Inst. Agri. Fish. Develop.)) ;
  • Kim, Gon-sup (College of Veterinary Medicine, Gyeonsang National University(Inst. Agri. Fish. Develop.)) ;
  • Hah, Dae-sik (Gyeongnam Provinical Government Institute of Health and Environment)
  • 김종수 (경상대학교 수의과대학(경상대학교 부속 농어촌 개발연구소)) ;
  • 김곤섭 (경상대학교 수의과대학(경상대학교 부속 농어촌 개발연구소)) ;
  • 하대식 (경남보건환경연구원)
  • Received : 1996.07.18
  • Published : 1997.03.25


A simple and rapid method known as matrix solid phase dispersion(MSPD) for simultaneous determination of 11 pesticide(2,4,5,6-tetrachlor m-xylene, ${\alpha}$-BHC, ${\gamma}$-BHC, ${\delta}$-BHC, aldrin, chlorfulazuron, heptachloroepoxide, dieldrine, endrin, endosulfan sulfate, and tetradifon) in beef fat was estabilished. Beef fat(0.5g) was fortified by adding the 11 pesticides and dibutylchlorendate as internal standard, and blended with 2g bulk $C_{18}$ in pestle and motar. Pesticides were eluted from an extraction column composed of $C_{18}$/ fat matrix blend and 2g activated Florisil by addition of 8ml acetonitrile. Then $2{\mu}l$ portion of the acetonitrile elute was directly analyzed by gaschromatography with electron capture detection. Unfortified blank control were treated similarly. Recovery rate were ranged from $83{\pm}5.4%$ to $94.2{\pm}7.6%$, intra-assay variability and inter-assay variability were ranged from 2.3% to 7.4%(n=5 for each insecticides) and from $6{\pm}1%$ to $12{\pm}3%$(n=10 for each insecticides), respectively. These results indicated that the MSPD methodology is aceptable for the extraction, determination and screening of residues 11 chroniated pesticides in beef fat.



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