국내에서 분리된 소 로타바이러스의 VP7 유전자 크로닝 및 염기서열 분석

Cloning and nucleotide sequence analysis of VP7 genes of bovine rotaviruses isolated in Korea

  • 강신영 (충북대학교 수의과대학) ;
  • 전성진 (충북대학교 수의과대학) ;
  • 장경옥 (오하이오주립대학교 수의과대학) ;
  • 박용하 (한국과학기술연구원 생명공학연구소) ;
  • 김원용 (중앙대학교 의과대학)
  • Kang, Shien-young (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeon, Seong-jin (College of Veterinary Medicine, Chungbuk National University) ;
  • Chang, Kyeong-ok (College of Veterinary Medicine, Ohio State University) ;
  • Park, Yong-ha (Korea Institute of Science and Technology, Genetic Engineering Research Institute) ;
  • Kim, Won-yong (College of Medicine, Chungang University)
  • 투고 : 1997.02.06
  • 발행 : 1997.06.25

초록

Bovine rotaviruses(A, 288, 55086 strains) isolated from fecal samples in Korea were propagated onto MA104 cells and were confirmed tentatively as G6, G8, and G10, respectively, by RFLP analysis. Full-length VP7 gene of these isolates was amplified by reverse transcriptase polymerase chain reaction(RT-PCR) using VP7 specific primers and cloned into TA vector. Nucleotide and deduced amino acid sequences of VP7 genes of the isolates were determined and compared with those of bovine rotavirus reference strains(NCDV; G6, UK; G6, Cody I-801; G8 and B223; G10). A, 288 and 55086 isolates showed high degree of nucleotide sequence homology with NCDV and UK(93% and 94%), Cody I-801(86%) and B223(97%), respectively, However, they showed 71~74% of nucleotide sequence homlogy with bovine rotavirus reference strains which belong to different serotypes. From the results of deduced amino acid sequence homology analysis, three isolates showed 94~96% of homology with the same serotype reference strains but 80~84% of homology with the different serotype reference strains. Three bovine rotavirus isolates, A, 288 and 55086 strains, were confirmed as G6, G8, and G10, respectively, by nucleotide and deduced amino acid sequence analysis.

과제정보

연구 과제 주관 기관 : 과학재단