The production of monoclonal antibodies against canine distemper virus

Canine Distemper Virus에 대한 단클론성 항체의 생산

  • Kim, Tae-jong (Department of Veterinary Medicine, College of Animal Husbandary and Animal Resources Center, Kon-Kuk University) ;
  • Kim, Se-young (Department of Veterinary Medicine, College of Animal Husbandary and Animal Resources Center, Kon-Kuk University)
  • 김태종 (건국대학교 축산대학 수의학과, 동물자원연구센타) ;
  • 김세영 (건국대학교 축산대학 수의학과, 동물자원연구센타)
  • Received : 1997.02.13
  • Published : 1997.06.25

Abstract

The purpose of the production of monoclonal antibodies aganist the Canine distemper virus(CDV) were perfect diagnosis and a new approach to treat canine distemper because the diagnosis and treatment of canine distemper were difficult. Canine distemper virus(CDV) was purified using saturated ammonium sulfate, and injected into hind footpads of BALB/c mouse. 12-15 days later, popliteal lymph node(PN) cells were harvested and fused with SP2/O myeloma cells. Characteristics of monoclonal antibodies were analysed. 1. 9 hybridomas produce the specific antibody against CDV. 2. 6 monoclonal antibodies are against intranuclear and cytoplasmic component of CDV, and 3 monoclonal antibodies are against cytoplasmic inclusions. 3. All monoclonal antibodies did not react with other 5 different viruses (CAV-I, CAV-II, CCV, CPV and CPIV) and react with another CDV-FXNO strain. 4. 3 monoclonal antibodies have neutralizing activity against CDV. 5. Antigenic difference was observed between CDV by IFA.