Development of Biosensor for Simultaneous Determination of Glucose, Lactic Acid and Ethanol

포도당, 젖산 및 에탄올의 동시 측정용 바이오센서의 개발

  • Kim, Jung-Ho (Department of Food & Microbial Technology, Seoul Women's University) ;
  • Rhie, Dong-Hee (Department of Electrical Engineering, The University of Suwon) ;
  • Kim, Tae-Jin (Department of Chemical Engineering, The University of Suwon) ;
  • Noh, Bong-Soo (Department of Food & Microbial Technology, Seoul Women's University)
  • 김정호 (서울여자대학교 식품.미생물공학과) ;
  • 이동희 (수원대학교 전기공학과) ;
  • 김태진 (수원대학교 화학공학과) ;
  • 노봉수 (서울여자대학교 식품.미생물공학과)
  • Published : 1998.02.01

Abstract

The purpose of this study is to develop biosensor for determination of glucose, lactate, and ethanol in foods and food-stuffs simultaneously. The multiple cathode system was prepared with an oxygen electrode having one anode and hexagonal cathode. Glucose oxidase, mutarotase, lactate oxidase, alcohol oxidase and catalase were used for immobilization to determine glucose, lactate, and ethanol. These components including ethanol were simultaneously determined by the immobilized enzymes in the multiple cathode system. The determination of the components by enzyme sensor was based on the maximum slope of oxygen consumption from enzyme reaction of each sensor part. The response time for analysis was 1 min. The optimum condition for glucose, lactate and ethanol sensor was found to be 0.1 M potassium phosphate buffer, pH 7.0 at $40^{\circ}C$. Interferences of various sugars and organic acids were investigated. Less than 10% of error was found in determination of the components except organic acids. This difference was compensated by the modified equation. This system was confirmed by conventional methods. It was concluded that the multiple cathode system of this study is for an effective method to determine sugar, organic acid, ethanol simultaneously in foods.

Keywords

multiple cathode system;simultaneous determination;glucose;lactic acid;ethanol