Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 30 Issue 1
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- Pages.22-34
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- 1998
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- 0367-6293(pISSN)
Development of Biosensor for Simultaneous Determination of Glucose, Lactic Acid and Ethanol
포도당, 젖산 및 에탄올의 동시 측정용 바이오센서의 개발
- Kim, Jung-Ho (Department of Food & Microbial Technology, Seoul Women's University) ;
- Rhie, Dong-Hee (Department of Electrical Engineering, The University of Suwon) ;
- Kim, Tae-Jin (Department of Chemical Engineering, The University of Suwon) ;
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Noh, Bong-Soo
(Department of Food & Microbial Technology, Seoul Women's University)
- Published : 1998.02.01
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82 5
Abstract
The purpose of this study is to develop biosensor for determination of glucose, lactate, and ethanol in foods and food-stuffs simultaneously. The multiple cathode system was prepared with an oxygen electrode having one anode and hexagonal cathode. Glucose oxidase, mutarotase, lactate oxidase, alcohol oxidase and catalase were used for immobilization to determine glucose, lactate, and ethanol. These components including ethanol were simultaneously determined by the immobilized enzymes in the multiple cathode system. The determination of the components by enzyme sensor was based on the maximum slope of oxygen consumption from enzyme reaction of each sensor part. The response time for analysis was 1 min. The optimum condition for glucose, lactate and ethanol sensor was found to be 0.1 M potassium phosphate buffer, pH 7.0 at
Keywords
multiple cathode system;simultaneous determination;glucose;lactic acid;ethanol