Cytotoxic Effect of the Pine needle extracts

솔잎 추출물의 in vitro계 암세포 성장억제효과

  • Kim, Eun-Jeong (Division of Food and Biotechnology, Kangwon National University) ;
  • Jung, Sung-Won (Division of Food and Biotechnology, Kangwon National University) ;
  • Choi, Keun-Pyo (Division of Food and Biotechnology, Kangwon National University) ;
  • Ham, Seung-Shi (Division of Food and Biotechnology, Kangwon National University) ;
  • Gang, Ha-Yeong (Forestry Research Institute)
  • 김은정 (강원대학교 식품생명공학부) ;
  • 정성원 (강원대학교 식품생명공학부) ;
  • 최근표 (강원대학교 식품생명공학부) ;
  • 함승시 (강원대학교 식품생명공학부) ;
  • 강하영 (임업연구원)
  • Published : 1998.02.01

Abstract

This study was performed to observe cytotoxic effect of the pine needle extracts against cancer cell lines including human gastric carcinoma (KATOIII), human lung carcinoma (A549), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) and SRB (sulforhodamine B) method. The extracts were prepared by step-wise fractionation of ethanol extract of pine needles using diethylether, chloroform, ethylacetate, butanol and water. The growth of the cancer cells in medium containing pine needle extracts were significantly inhibited degree in proportion to the increase of the extract concentration. A significant shrinkage of Hep3B cells was observed when the cells were exposed into 0.5, 1 mg/mL of pinus rigida extract.

Keywords

pine needle extracts;cytotoxic effect;cancer cell lines;MTT;SRB;morphology