Screening of HMG-CoA Reductase Inhibitory Activity of Ethanol and Methanol Extracts from Cereals and Regumes

곡류 및 두류 추출물로 부터 HMG-CoA reductase 저해활성 검색

  • Ha, Tae-Youl (Korea Food Research Institute, Rice Research Group) ;
  • Cho, Il-Jin (Korea Food Research Institute, Rice Research Group) ;
  • Lee, Sang-Hyo (Korea Food Research Institute, Rice Research Group)
  • 하태열 (한국식품개발연구원 쌀연구사업단) ;
  • 조일진 (한국식품개발연구원 쌀연구사업단) ;
  • 이상효 (한국식품개발연구원 쌀연구사업단)
  • Published : 1998.02.01

Abstract

A study was conducted to screen the inhibitory activity of 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA) reductase, which is known to be rate-limiting enzyme in cholesterol bosynthesis, from the extracts of 80% methanol and 70% ethanol of cereals and regumes. The strongest inhibitory activity was shown in the ethanol extract of sorghum among the ethanol extracts. The inhibitory activity of HMG-CoA reductase of prosomillilet methanol extract was 73%, and highest among the methanol extracts. The inhibitory activity of 44.7% was observed in sorghum methanol extract. The methanol extracts of prosomillet and sorghum were further fractionated with hexane, chloroform, ethylacetate, butanol and water. HMG-CoA reductase inhibitory activity was shown in all fractions of prosomillet and sorghum methanol extracts. Hexan fraction of both prosomillet and sorghum had the strongest inhibitory activity among five fractions, and the inhibitory activity was increased compared to each crude extracts.

Keywords

HMG-CoA reductase inhibitor;cereals;methanol extract