Isolation of an Anticariogenic Compound from Magnoliae Bark

후박피(Magnoliae bark)로부터 항충치활성을 갖는 물질의 분리

  • Published : 1998.02.01

Abstract

We have screened total 32 herbal drugs to find the highest activity against human cariogenic enzyme, glucosyltransferase (GTase) from the extracts of Magnoliae bark. The extracts were separated into three phases, i. e. water, n-butanol and ethylacetate according to their solvent polarity. Among them, ethylacetate fraction had approximately more than 70% of total activities, and the active principle was further isolated by prep. HPLC following silicagel column chromatography to yield single compound as white powder. The chemical structure of the compound was finally elucidated to be 4,4'-dihydroxy-3,3'-dimethoxylignan from the spectral data of FAB-MS. $^1H-\;and\;^{13}C-NMR$ spectrometries. The compound was also shown to have relatively strong antibacterial activity against ten types of cariogenic oral bacteria and one kind of Actinomyces sp.

Keywords

Magnoliae bark;Streptococcus mutans;glucosyltransferase;4,4'-dihydroxy-3,3'-dimethoxylignan