Inhibitory Effect of Main Pine Needle Extracts on the Chemically Induced Mutagenicity

주요 솔잎 추출물의 돌연변이 억제효과

  • Kim, Eun-Jeong (Division of food and Biotechnology, Kangwon national university) ;
  • Jung, Sung-Won (Division of food and Biotechnology, Kangwon national university) ;
  • Choi, Keun-Pyo (Division of food and Biotechnology, Kangwon national university) ;
  • Ham, Seung-Shi (Division of food and Biotechnology, Kangwon national university) ;
  • Kang, Ha-Young (Forestry Research Institute)
  • 김은정 (강원대학교 식품생명공학부) ;
  • 정성원 (강원대학교 식품생명공학부) ;
  • 최근표 (강원대학교 식품생명공학부) ;
  • 함승시 (강원대학교 식품생명공학부) ;
  • 강하영 (임업연구원)
  • Published : 1998.04.01

Abstract

Pine has been known as a traditional medicinal plant and as showing a physically beneficial function to a human being. Therefore, this study was performed to investigate the physiological activities of main pine neddles. Ethanol extracts from pinus needles did net exhibit any mutagenicity. On the contrary, inhibitory effects of ethanol extract were observed on mutagenicity induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), 4-nitroquinoline-1-oxide (4NQO), 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1) and benzo(a)pyrene $(B({\alpha})P)$ using Salmonella typhimurium reversion assay. On direct-acting mutagen (MNNG, 4NQO) and indirect-acting mutagen (Trp-P-1, $(B({\alpha})P)$, we observed higher inhibitory effect. Stepwise fractionation of the ethanol extract was done by using ether, chloroform, ethyl acetate, butanol and water to obtain effective fraction. Among them, water fractions $(100\;{\mu}g/plate)$ of Pinus thunbergii, Pinus rigida, Pinus densiflora and Pinus koraiensis showed high inhibition of 91.65%, 94.7%, 84.22% and 79.02%, respectively, on the mutagenicity of MNNG in Salmonella typhimurium TA100.

Keywords

pine needles;spore rec-assay;Salmonella typhimurium reversion assay;antimutagenicity