Bio-antimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS Chromotest

SOS Chromotest에서 숙지황 물 추출물의 세포내 항돌연변이 효과

  • Ahn, Byung-Yong (Division of Life Science and Natural Resource, Wonkwang University) ;
  • Lee, Kap-Sang (Division of Life Science and Natural Resource, Wonkwang University) ;
  • Maeng, Il-Kyung (Department of Food Science and Technology, Chonbuk National University) ;
  • Song, Geun-Seoub (Department of Food Engineering, Iri National College of Agriculure & Technology) ;
  • Choi, Dong-Seong (Division of Food, Enviromental and Chemical engineering and Biotechnology, Woosuk University)
  • 안병용 (원광대학교 생명자원과학부) ;
  • 이갑상 (원광대학교 생명자원과학부) ;
  • 맹일경 (전북대학교 식품공학과) ;
  • 송근섭 (이리농공전문대학 식품공업과) ;
  • 최동성 (우석대학교 생물식품 및 환경화공학부)
  • Published : 1998.04.01

Abstract

The antimutagenic activity of the water extract of Rehmannia glutinosa Liboschitz (RG) on the mutagenicity induced by 4-nitroquinoline 1-oxide (4-NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), $aflatoxin\;B_1\;(AFB_1)$ and benzo(a)pyrene [B(a)P] were studied using the SOS Chromotest with Escherichia coli PQ37. The water extract of RG was separated into methanol soluble and methanol insoluble parts. The methanol soluble part exhibited higher inhibition effects than the methanol insoluble part against the mutagenic activities of five mutagens. Step-wise fractionation of methanol soluble part was done using methanol, ethyl acetate and water. Among these fractions, water fraction had the strongest inhibitory effects against the mutagenenicity of five model mutagens, showing $4.5{\sim}29.5%$ inhibition, but the $AFB_1$ mutagenic potency was increased slightly by ethyl acetate fraction. The water fraction was further partitioned by sephadex LH-20 column chromtography, and 9 subfractions were obtained. The fraction III showed the strongest inhibitory effects with dose response against the mutagenic activities induced by all the tested chemical mutagens. The inhibition rates of fraction III at concentration of $400\;{\mu}g/assay$ were 29%, 35%, 38%, 25% and 24% against 4-NQO, MNNG, MMC, AFBl and B(a)P, respectively. The fraction III also exhibited a strong bio-an-timutagenicity against 4-NQO and $AFB_1$ by showing more than 40% inhibition.

Keywords

Rehmannia glutinosa Liboschitz;SOS chromotest;bio-antimutagenic effects;dose response