Production of Monoclonal Antibody against Zearalenone Produced by Fusarium sp.

Fusarium sp.가 생성하는 zearalenone에 대한 단크론성 항체생산

  • Kang, Sung-Jo (Institute of Agriculture and Fishery Development, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Department of Food Science and Technology, Gyeongsang National University) ;
  • Kang, Jin-Soon (Department of Home Economic, Chinju Technical college)
  • 강성조 (경상대학교 농어촌개발연구소) ;
  • 정덕화 (경상대학교 식품공학과) ;
  • 강진순 (진주전문대학 가정과)
  • Published : 1998.12.01

Abstract

To develop zearalenone-specific monoclonal antibodies, hybridoma cells were produced by fusion of myeloma cells $(P3{\times}63Ag\;V653)$ and spleen cells from BALB/c female mice immunized with zearalenone-oxime coupled to bovine serum albumin (BSA). After screening of antibody titer of them with a sandwich type enzyme-linked immunosorbent assay (ELISA), 5 hybridomas which could produced monoclonal antibodies with a high affinity for zearalenone were selected. The monoclonal antibody produced by Z-2-M26 hybridoma exhibited the high sensitivity to zearalenone and a little cross-reactivity to ${\alpha}-zearalenol$ (11%), but did not react with ${\beta}-zearalenol,\;{\alpha}-zearalenol,\;{\beta}-zearalenol$ and DON. In conclusion, the developed monoclonal antibody appeared to be a very promising immunoreagent for the future development of a specific and sensitive quantitative ELISA for zearalenone.

Keywords

monoclonal antibody;zearalenone;hybridoma;myeloma cell