Screening of Ochratoxin A Producing Fungi from Greenhouse Horticulture

시설원예산물로부터 Ochratoxin A 생성 곰팡이의 검색

  • Kang, Sung-Jo (Institute of Agriculture and Fishery Development) ;
  • Park, Bong-Jung (Department of Food Science and Technology, Gyeongsang National University) ;
  • Lee, Jong-Ok (Division of Food Standard, Korea Food and Drug Administration) ;
  • Kang, Jin-Soon (Department of Home Economic, Chinju Technical College) ;
  • Chung, Duck-Hwa (Department of Food Science and Technology, Gyeongsang National University)
  • 강성조 (경상대학교 농어촌개발연구소) ;
  • 박봉정 (경상대학교 식품공학과) ;
  • 이종옥 (식품 의약품 안전청 식품규격과) ;
  • 강진순 (진주전문대학 가정학과) ;
  • 정덕화 (경상대학교 식품공학과)
  • Published : 1998.12.01

Abstract

In order to evaluate the safety of greenhouse horticultures, a large number sample sources were collected, and the fungi of Aspergillus sp. and Penicillium sp. were isolated from them. Indirect competitive ELISA method and high performance liquid chromatography (HPLC) were applied to confirm the ochratoxin A producing abilities of isolated strains. One hundred ninety two sample sources including soil, pepper, strawberry and water mellon were collected for fungi isolation from western Gyeongnam, Andong and Gyeongbok. One hundred forty two strains of Aspergillus sp. and one hundred fifty three strains of Penicillium sp. were isolated respectively from them. The isolated fungi were tested for the production of ochratoxin A by ELISA. After culture of them on the modified sucrose low salt medium at $28^{\circ}C$ for 15 days, we found that five strains of Penicillium sp. produced ochratoxin A at the levels of $0.084{\sim}2.128\;{\mu}g/mL$. Among them, #129-2 strain isolated from water melon, showed the highest level of ochratoxin A as $2.128\;{\mu}g/mL$ broth. However, all of isolated Aspergillus sp. didn't produce ochratoxin A. When we compared the results of ELISA method with HPLC method, ochratoxin A production of each isolated strains showed very similar levels.

Keywords

Ochratoxin A;greenhouse horticulture;indirect competitive ELISA