Antimicrobial Characteristics of Chitosan and Chitosan Oligosaccharides on the Microorganism related to Kimchi

키토산 및 키토산 올리고당의 김치 관련 미생물에 대한 항균 특성

  • Published : 1998.12.01


Antimicrobial activity of chitosan and chitosan oligosaccharides on the microorganism related to Kimchi was investigated. 5 kinds of chitosan, which have different deacetylation degrees and molecular weights were prepared and its effect on the organoleptic characteristics, pH and titrable acidity of Kimchi in the storage time were examined. C-4 and C-5 chitosan (D.A.:$92{\sim}99%$, M.W.: $16,000{\sim}32,000)$ recorded high score in the texture and showed pH 4.9 and titrable acidity 0.35%, compared with control (pH 4.1, titrarable acidity 0.50 %) evaluated to optimal ripening time. The chitosan oligosaccharides containing relatively large amount of $pentamer{\sim}heptamer$ were chosen from C-4 chitosan hydrolyzates. Antimicrobial activity of C-4 and chitosan oligosaccharides against B. subtilis, B. cereus, Pse. fluorescens, E. coli, Lac. plantarum, Leu. mesenteroides, Lac. brevis, Ent. faecalis and 3 kinds of microflora from Kimchi were examined. The clear zone against microorganism were $9{\sim}20mm$ at 3.0% C-4 chitosan and $8{\sim}24mm$ at 5.0% chitosan oligosaccharides, and MIC of chitosan and chitosan oligosaccharides was shown $0.01{\sim}0.05%$ and $0.05{\sim}0.2%$, respectively. The antimicrobial effect of chitosan and chitosan oligosaccharides was also observed in 3 kinds of total microflora from Kimchi and was most strong in the microflora from the ripening stage of Kimchi, suggesting C-4 chitosan and chitosan oligosaccharides could be applicable to extending shelf-life of Kimchi.