Mutagenicity of Chloropropanols in SOS Chromotest and Ames Test

SOS Chromotest 및 Ames test에서의 Chloropropanol류의 변이원성

  • Song, Geun-Seoup (Department of Food Engineering, Iri National College of Agriculture and Technology) ;
  • Han, Sang-Bae (Seoul FDA) ;
  • Uhm, Tae-Boong (Faculty of Biological science, Chonbuk National University) ;
  • Choi, Dong-Seong (Division of Biotechnology and Environmental engineering, Woosuk University)
  • 송근섭 (이리농공전문대학 식품공업과) ;
  • 한상배 (식품의약품안전청 서울지방청) ;
  • 엄태붕 (전북대학교 생물과학부) ;
  • 최동성 (우석대학교 환경생명공학부)
  • Published : 1998.12.01

Abstract

SOS Chromotest and Ames test were carried out to evaluate the mutagenicity of three chloropropanols. In the SOS Chromotest, 3-monochloro-l,2-propanediol (3-MCPD) and 2,3-dichloro-1-propanol (2,3-DCP) except for 1,3-dichloro-2-propanol (1,3-DCP) induced SOS response in Escherichia coli PQ37 with dose-response relationship and 2,3-DCP was far more genotoxic than 3-MCPD. The genotoxic activities of both compounds, however, were very lower in E. coli PQ35 (PQ37 $uvrA^+)$ as compared to them in E. coli PQ37, whereas much higher in E. coli PQ243 (PQ37 tagA alkA). These results indicate that there are at least two types of DNA lesions caused by these compounds; one is a excision-repairable and the other is 3-methyladenine or any similar lesion which is excision-unrepairable and can induce adaptive response. In Salmonella typhimurium TA100, all the compounds showed strong mutagenicities, establishing the following genotoxic order: 2,3-DCP>3-MCPD>1,3-DCP. But the mutagenic activities were very low in S. typhimurium TA98 and TA97a. These results suggest that the mutation by chloropropanols can be induced by the DNA lesions causing base-pair substitutions. From the result that the mutagenicities of 3-MCPD and 2,3-DCP in S. typhimurium TA1535 were very low as compared to those in S. typhimurium TA100, it was appeared that the mutations by both compounds necessitate error-prone SOS repair.

Keywords

chloropropanols;mutagenicity;Ames test;SOS chromotest