Effect of Korean Rice-Wine (Yakju) on in vitro and in vivo Progression of B16BL6 Mouse Melanoma and HRT18 Human Colon Adenocarcinoma Cells

한국 전통 약주의 B16BL6 mouse melanoma 및 HRT18 human colon adenocarcinoma 세포 성장 억제 효과

  • Chung, Kun-Sub (Department of Biological Resources and Technology, Yonsei University) ;
  • Oh, Won-Taek (Korea Institute of Food Hygiene) ;
  • Nam, Sang-Min (Department of Medical Technology, Yonsei University) ;
  • Son, Byoung-Soo (Department of Medical Technology, Yonsei University) ;
  • Park, Yong-Serk (Department of Medical Technology, Yonsei University)
  • 정건섭 (연세대학교 생물자원공학과) ;
  • 오원택 (한국식품위생연구원) ;
  • 남상민 (연세대학교 임상병리과) ;
  • 손병수 (연세대학교 임상병리과) ;
  • 박용석 (연세대학교 임상병리과)
  • Published : 1998.12.01

Abstract

Two kinds of Korean rice-wine (Yakju) with different process and ingredients, and Japanese rice-wine (Sake) were chosen for this study, and throughly dried and solubilized in water or cell culture medium. In vitro cytotoxicity assays of the solubilized wine solids exhibited that maximum dilution factors for inhibition of B 16BL6 mouse melanoma cell growth were 16X for herbal medicine-added rice-wine (Korean rice-wine I) and typical Korean rice-wine (Korean rice-wine II), and 8X for Japanese rice-wine. Their cytotoxic effects on HRT18 human colon adenocarcinoma cells were even lower than those on B16BL6 cells. The morphology of the tumor cells were changed by addition of the solubilized wine solids. Inhibitory effect of the rice-wine on in vivo tumor growth and metastasis were monitored after implantation of B16BL6 cells into C57BL/6 mice with daily feeding the solubilized wine solids. Compared to non-fed control groups, B16BL6 tumor growth and metastasis to lung were clearly inhibited by feeding the wine solids, in order of Korean rice-wine I > Korean rice-wine II > Japanese rice-wine. The data of in vitro cytotoxicity and the cell shape changes indicate that the inhibitory effect of tumor progression may be attributed to tumor cell differentiation or immune stimulation induced by certain components in the rice-wine, rather than direct cytotoxicity of the components.

Keywords

rice wine;yakju;sake;cancer;cytotoxicity