Purification and Characterization of Cyclodextrin Glucanotransferase from Bacillus stearothermophilus KJ16

Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrin Glucanotransferase 의 정제와 효소특성

  • Kwon, Hyun-Ju ;
  • Nam, Soo-Wan ;
  • Kim, Kwang-Hyun ;
  • Song, Seong-Koo ;
  • Yun, Jong-Won ;
  • Kim, Byung-Woo
  • 권현주 (동의대학교 미생물학과) ;
  • 남수완 (동의대학교 미생물학과) ;
  • 김광현 (동의대학교 미생물학과) ;
  • 송승구 (부산대학교 화학공학과) ;
  • 윤종원 (대구대학교 생물공학과) ;
  • 김병우 (동의대학교 미생물학과)
  • Published : 1998.06.01

Abstract

Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purifice enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and $60^{\circ}C$, respectively. The enzyme was stable at $50^{\circ}C$ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the $^{\alpha}$, $^{\beta}$, $^{\gamma}$-CD ratio of 42:46:12. Amylopectin was the most suitable substrate with 67% conversion to CD.

Keywords

References

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