The Physicochemical Properties of $\alpha$-Amylase Inhibitors from Black Bean and Naked Barey in Korea

한국산 검정콩 및 쌀보리 $\alpha$-Amylase 저해물질의 이화학적 특성

  • Published : 1998.06.01


The physicochemical properties of the $\alpha$-amylase inhibitors from black bean and naked barley is Korea were investigated. Preincubation time for maximum inhibition was 30min and no activity change was seen after that time. Optimum pH of the $\alpha$-amylase inhibitors from the black bean and naked barley was pH 7.0 and the inhibitory activities were stable in the range of pH 6.0~8.0 in both phosphate and Tris-HCI buffer solutions. Both inhibitors maintained more than 50% of activity after incubation for 17 min at 7$0^{\circ}C$. The inhibitors from the black bean and naked barley maintained more than 50% of activities after treatment for 40 min and 30 min with pepsin, and 30 min and 50 min with trypsin, respectively. Both inhibitors functioned via a noncompetitive mechanism and were active against porcine pancreatic and human salivary $\alpha$-amylases. The activities of both inhibitors were linear for the ionic stength ranging from 0 to 0.9. The addition of 70 mM maltose to the reaction mixture caused a maximum increase in the relative activities of both inhibitors, but it did not affect the dissociation of the EI complex. The activities of both inhibitors were significantly enhanced by adding 1mM of K+ or Mg2+.


  1. J. Agric. Food Chem. v.33 Isolation and characterization of a trypsin/chymotrysin inhibitor from the Millet Eshinocloa fruneutacea Dupa, S. L.;Pattabiraman, T. N.
  2. Agric. Biol. Chem. v.49 Inhibitory properties of an α-amylase inhibitor, Pain, from Streptomycs corchorushii Arai, M.;Oouchi, N.;Murao, S.
  3. Diabetologia v.9 Influence of α-amylase inhibitor(BAYD7791) on blood-glucose, serum-insulin and NRFA in starch loading test in rats, dogs and man Puls, W.;Keup, U.
  4. Chem. Abstr. v.27 "Sisto-amlyase", a natural inhibitor of amylase Chrzaszcz, T.;Janicki, J.
  5. Phytochemistry v.20 The complete amino acid sequence of a major wheat protein inhibitor of α-amylase Kashland, N.;Richardson, M.
  6. J. Food Biochem. v.2 Purification and characterization of an amylase inhibitor from rye(Secale cerale) flour Granum, P. E.
  7. Science v.102 Amylase inhibitor of navy beans Bowman, D. E.
  8. J. Sci. Food Agric. v.37 The effects of α-amylase inhibitors on insect storage pestst L The Inhibition of α-amylase in vitro and effects on development in vivo Gatehouse, A. M. R.;Fenton, K. A.;Pavey. D. J.
  9. J. Csrsal Sci. v.9 Accumulation of an endogenous α-amylase inhibitor in barley during grain development Robertson, M;Hill, R. D.
  10. Die Starke. v.27 Assay of alpha-amylase inhibitor activity in legumes Marshall, J. J;Lauda, C. M.
  11. J. Sci. Food Agric. v.57 Variation in protein and trypsin of winged bean(Prophocarpus tetragonolobus) seeds Prakash, D.;Misra, P. N.;Misra, P. S.
  12. J. Food Biochem. v.8 Purification and partial characterization of two α-amylase inhibitors from black bean(Phaseolus vulgaris) Frels, J. M.;Rupnow, J. H.
  13. Physiol. Chem. v.359 Proteinaceous α-amylase inhibitor from beans(Phaseolus vulgaris). Purification and characterization Pick, K. H.;Wobsr, G.
  14. J. Nutr. v.94 Heat-labile growth-inhibiting factors in bean(Phaseolus vulgaris) Jaffe, W. G.;Lette, L. V.
  15. Mol. Cell. Biochem. v.18 A Model for the interaction of wheat monomeric and dimetric protein inhibitors with α-amylase Silano, V.;Posrio, E.;Buonocore, V.
  16. Food Chem. v.34 α-Amylase inhibitors in foodstuffs Udupa, S. L.;Prabhakar, A. R.;Tandon, S.
  17. Kur. J. Appl. Microbiol. v.13 Screening and classification of actinomycetes produccing α-amylase inhibitors and the isolation, their kinetic studies of α-amylase inhibitors Kim, J. K.;Kim, J. W.;Kim, H. W.;Shim, M. J.;Dhoi, E. C.;Kim, B. K.
  18. Korean J. Food Sci. Technol. v.20 Screening of oriential drugs for α-amylase inhibitor Lee, K. S.;Yang, C. B.
  19. J. Korean Soc. Food Nutr. v.24 Purification of α-amylase inhibitor from naked barley in Korea Moon, J. S.;Shin, C. S.;Choi, J. S.;Park, S. K.;Shim, K. H.
  20. J. Food Sci. v.50 Characterization and two α-amylase inhibitior from black been Phaseolus vulgaris Frels, J. M.;Rupnow, J. H.
  21. J. Food Biochem. v.5 Purification and characterization of α-amylase inhibitor from maize (Zea maize) Blanco-Labra, A.;Iturbe-Chinas, F. A.
  22. J. Food Biochem. v.2 Purification and characterization of an amlyase inhibitor from rye(Secale creale) flour Granum, P. E.
  23. Sitophilus oryzae. Insect Biochem. v.18 Purification of an α-amylase inhibitor from wheat, Triticum aestivum, and its interaction with amylases from the rice weevil Baker, J. E.
  24. Comp. Biochem. Physiol. v.93B Interaction of partially-purified amlyases from larval anagasta kuehniella(Lepidoptera : pyralidae) with amylase inhibitors from whieat Baker, J. E.
  25. Biochem. Biophys. Acta. v.221 α-Amylase inhibitors from wheat isolation and characterization Shainkin, R.;Birk, Y.
  26. Biochem. Biophy. Acta. v.658 Isolation and characterization of four inhibitors from wheat flour which display differential inhibition specificites for human salivary and human pancreatic α-amylase O'Connor, C. M.;Mcgeeney, K. F.
  27. Biochem. Biophy. Acta. v.422 Purification and properties of an α-amylase inhibitor from wheat O'Donnell, M. D.;McGeeney, K. F.
  28. J. Food Sci. Technol. v.27 Purification of α-amylase inhibitor from black bean in Korea Moon, J. S.;Bae, Y. I.;Shim, K. H.