Levan으로부터 Levanbiose를 생산하는 미생물의 분리 및 배양

Isolation and Cultivation of Microorganism Producing Levanbiose from Levan

  • 발행 : 1998.06.01


A bacterial strain No. 43 was isolated from soil samples as a levan-assimiating microorganism producing an extracellular levanase and hydrolying levan to levanbiose. According to the taxonomic characteristics of its morphological and physiological properties, the strain was idenified as Pseudomonas sp. No. 43. The optimum culura medium was composed of 10g levan, 5g(NH4)2SO4, 3g NH4Cl, 3g polypepton, 1g K2HPO4, 0.5gMgSO4.7H2O, and 0.2g MnCl2.4H2O per liter. The cultivation for levanase was carried out in pH 7.0 at 4$0^{\circ}C$ for 28hr. The reaction product was a kind of oligosaccharide and it was purified by chilled ethanol precipitation and gel filtration for evalluation of degree of polymerization (DP). The purified product was determined as levanbiose of MW 342 and DP2 by HPLC and FAB-MS.


  1. Microflora v.5 Bifidobacteria Hidaka, H.;Eida, T.;Takizawa, T.;Tokunnaga, T.;Tashiro. Y.
  2. Agric. Biol. Chem. v.52 A fructooli-gosaccharide-producing enzyme from Asperfillus niger ATCC 20611 Hidaka, H.;Hirayama, M.;Sumi, N.
  3. Biosci. Biotech. Biochem. v.57 Purification and properties of β-1,4-xylanases 2and 3 from Aeromonas caviae W-61 Dung, N. Y.;Vetayasuporn, S.;Kamio, Y.;Abe, N.;Kaneko, J.;Izaki, K.
  4. Appl. Microbiol. Biotechnol. v.26 Purification, properties and comparision of invertase, exoinulinase and endoinulinase of Aspergillus ficcum Ettalibi, M.;Baratti, J. C.
  5. J. Ferment. Bioeng. v.67 Purification and some properties of endoinulinase from Chrysogenum pannorum Xiao, R.;Tania, M.;Takao, S.
  6. Biosci. Biotech. Biochem. v.57 Purification and characterization of β-fructofuranosidase from Aspergillus japonicus Duan, K. J.;Sheu, D. C.;Chen, J. S.
  7. Biosci. Biotech. Biochem. v.57 Purification and some properties of β-fructofurabosidase from Bifidobacterium adolescentis G1 Muramatsu, K.;Onodera, S.;Kikuch, M.;Shiomi, N.
  8. J. Polymer Science v.23 The structure and properties of levan, a polymer of D-fructose produced by culture and cell free extracts of Aerobacter levanicum Freingold, D. S.;Gehatia, M.
  9. J. Ferment. Bioeng. v.75 Characterization of levan produced by Serratia sp. Ichiro, K.;Saito, T.;Iizuka, M.;Minamiura, N.;Ono, S.
  10. Agric. Biol. Chem. v.54 Purification and some properties of a levancase from Streptomyces sp. No.7-3 Murakami, H.;Mukauo, H.;Kitahata, S.
  11. Biosci. Biotech. Biochem. v.56 Purification and some properties of a new levanase from Bacillus sp. No. 71 Murakami, H.;Kuramoto, H.;Mizutani, K.;Nakano, H.;Kitahata, S.
  12. J. Biochem. v.90 Enzymatic formation of difructose anhydride Ⅳ from bacterial levan Tanaka, K.;Kawaguchi, H.;Ohno, K.;Shohji, K.
  13. Biochem. J. v.98 Sucrose utilization by Zymomonas mobilis Dawes, E. A.;Ribbons, D. W.
  14. Bergy's manual of systematica bacteriology v.4 Standely, T. W.;Sharpe, M. E.;Holt, J. G.
  15. Bergy's manual of determinative bacteriology(9th ed.) John, G.;Krieg, N. R.;P. H. A. Staley, J. T.;Williams, S. T.
  16. Biosci. Biotech. Biochem. v.57 Poduction of levanbiose by a levan-degrading enzyme from Streptomyces exofoliatus F3-2 Yokota, A.;Kondo, K.;Nakagawa, M.;Kojima, I.;Tomia, F.