Antitumor Evaluation of Epigallocatechin Gallate by Colorimetric Methods

비색분석법에 의한 Epigallocatechin Gallate의 항암효과평가

  • Baek, Soon Ok (Chemical Analysis Center, Korean Ginseng & Tobacco Research Institute) ;
  • Kim, Il Kwang (Department of Chemistry, Wonkwang University) ;
  • Baek, Seung Hwa (Department of Chemistry, Wonkwang University) ;
  • Han, Du Seok (Department of Oral Anatomy, Wonkwang University)
  • 백순옥 (한국인삼연초연구소 분석센터) ;
  • 김일광 (원광대학교 자연과학대학 화학과) ;
  • 백승화 (원광대학교 자연과학대학 화학과) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실)
  • Published : 19980800

Abstract

In the present study, we were evaluated cytotoxic effects of epigallocatechin gallate in human skin melanoma cells such as HTB-69. The light microscopic study showed morphological changes of the treated cells. Disruptions in cell organelles were determined by colorimetric methods; 3-(4,5-dimethyl thiazol-2-yl)-2,5diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhodamine B protein (SRB) assay. These results suggest that epigallocatechin gallate retains a potential antitumor activity.

Keywords

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