Characterization of a heat-resistant antimicrobial peptide secreted by Bacillus subtilis A405

Bacillus subtilis A405 균주가 생성하는 내열성 항균 peptide의 특성 검정

  • Koo, Bon-Sung (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Seung-Bum (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoon, Sang-Hong (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Song, Gae-Kyung (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Chung, Dae-Sung (Dept. of Postharvest Technology, National Horticulture Research Institute, RDA) ;
  • Byun, Myung-Ok (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Ryu, Jin-Chang (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA)
  • 구본성 (농업과학기술원 생물자원부 분자유전과) ;
  • 이승범 (농업과학기술원 생물자원부 분자유전과) ;
  • 윤상홍 (농업과학기술원 생물자원부 분자유전과) ;
  • 송재경 (농업과학기술원 생물자원부 분자유전과) ;
  • 정대성 (원예연구소 저장이용과) ;
  • 변명옥 (농업과학기술원 생물자원부 분자유전과) ;
  • 류진창 (농업과학기술원 생물자원부 분자유전과)
  • Published : 1998.12.30

Abstract

An antimicrobial peptide producing bacterium, Bacillus subtilis A405, was screened and identified among 700 of antagonistic bacteria. The heat-resistant antimicrobial peptide, AMP-405, was purified from the broth culture of B. subtilis A405 through $20{\sim}40%$ ammonium sulfate precipitation and ultrafiltration. The AMP-405 exhibited strong antimicrobial activities against Botrytis cinerea, Cercospora sp., Fusarium oxysporum, Penicillium digitatum, Celletotrichum gloeosporioides, Rhizoctonia solani, Pythium ultimum, Pyricularia oryzae, Escherichia coli, Pseudomonas spp. and Candida albicans. The molecular weight of the peptide was about 3.0 kDa determined by SDS-PAGE, Native-PAGE and Tris-Tricine gradient electrophoresis, and composed of 9 kinds of amino acid such as aspartic acid, glycine, serine, glutamine, valine, leucine, isoleucine, proline, tyrocine. To determine the efficiency of AMP-405 as a potential maintenance of fruits freshness, cherry tomato was srored at $25^{\circ}C$ for 2 weeks after treatment with $50{\mu}g/ml$ of AMP-405 and $10^{5}$ spores/ml of Botrytis cinerea simultaneously. Treatment with AMP-405 resulted in significantly less infection by Botrytis cinerea, than the treatment with tap water as a control.