Determination and confirmation of the carbendazim residue in soybean sprout

콩나물중 살균제 carbendazim 잔류분의 정량 및 확인

  • Kim, Young-Gook (Agriculture & Livestock Products Analysis Division, Health and Environment Institute of Chollanam-do) ;
  • Park, Jong-Tae (Agriculture & Livestock Products Analysis Division, Health and Environment Institute of Chollanam-do) ;
  • Hong, Suk-Soon (Agriculture & Livestock Products Analysis Division, Health and Environment Institute of Chollanam-do)
  • 김영국 (전라남도보건환경연구원 농축산물분석과) ;
  • 박종태 (전라남도보건환경연구원 농축산물분석과) ;
  • 홍석순 (전라남도보건환경연구원 농축산물분석과)
  • Published : 1998.12.30

Abstract

Tandem HPLC and atmospheric pressure chemical ionization(APcI) LC/MS method was used for the determination and confirmation of carbendazim residues in soybean sprout. Fluorescence(FL) detector was connected in tandem with the ultraviolet(UV) detector for dual detection of the carbendazim residue at the excitation and emission wavelength of 280 nm and 310 nm, respectively. The limit of detection for carbendazim was $0.1{\mu}g/kg$ sample. Recoveries of carbendazim from fortified soybean sprout at 0.5, 1.0 and 2.0 ppm were averaged 89.1%. Mass spectrometry using a APcI source confirmed the carbendazim residue in the soybean sprout sample. Fragmentation pattern on the APcI LC/MS spectrum of carbendazim was simpler than that from electron impact(EI) mass spectrum. Carbendazim produced 3 major ions including m/z 133, m/z 159 and m/z 191($M^{+}$). This method was sensitive enough to provide reliable and reproducible results for practical applications.