In vivo metabolism of carbofuran in resistant and susceptible brown planthoppers, Nilaparvata lugens $St{\aa}l$

저항성 및 감수성 벼멸구 체내에서의 카보후란 대사

  • Yoo, Jai-Ki (National Institute of Agricultural Science and Technology, RDA) ;
  • Ahn, Yong-Joon (Division of Applied Biology and Chemistry and the Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Shono, Toshio (Applied Zoology Lab., Institute of Agriculture and Forestry, University of Tsukuba) ;
  • Lee, Si-Woo (National Institute of Agricultural Science and Technology, RDA)
  • 유재기 (농업과학기술원) ;
  • 안용준 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 정야준부 (일본츠쿠바대학 응용동물학실) ;
  • 이시우 (농업과학기술원)
  • Published : 1998.09.30

Abstract

This study was conducted to find out the biochemical or metabolic resistance mechanism of brown planthopper (BPH) to carbofuran. Differences between resistant ($LD_{50};\;20.3{\mu}g/g$) and susceptible strains($LD_{50};\;0.3{\mu}g/g$) were shown. The amounts of carbofuran metabolite, benzofuranol, and the origin, not developed by Thin Layer Chromatography, were much more in the susceptible strain. But the mother compound, carbofuran, was much more in the resistant strain. The tendencies of metabolism one and three hours after treatment were similar in both strains except for the amounts of metabolites described above. From the study, it is supposed that hydrolytic enzyme, esterase, changes its role from cleaving the esteric bond of carbofuran to making conjugates with carbofuran. This seems to be the main resistance mechanism of BPH to carbofuran. Oxidase and transferase may play little or no role in resistance mechanism. Oxidative and transferring enzymes gave no effects on the metabolism of carbofuran in the resistant strain compared with the susceptible strain.