Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography

역상 고속 액체크로마토그래피를 이용한 인삼 사포닌의 분석

  • Received : 1998.06.19
  • Published : 1998.12.25

Abstract

Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak $C_{18}$ ODS column ($3.9mm{\times}150mm$, $5{\mu}m$), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf and $Rg_1$ was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.

Keywords

ginsenoside;saponin;reverse-phase high performance liquid chromatography