Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography

역상 고속 액체크로마토그래피를 이용한 인삼 사포닌의 분석

  • 정승일 (원광대학교 자연과학대학 화학과) ;
  • 김천석 (한국인삼연초연구원) ;
  • 이노운 (한국인삼연초연구원) ;
  • 최강주 (한국인삼연초연구원) ;
  • 이용구 (한국화학연구소) ;
  • 김일광 (원광대학교 자연과학대학 화학과)
  • Received : 1998.06.19
  • Published : 1998.12.25


Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak $C_{18}$ ODS column ($3.9mm{\times}150mm$, $5{\mu}m$), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf and $Rg_1$ was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.