Preparation of Standard Phosphatidylcholine Hydroperoxide for Chemiluminescence-HPLC Assay

화학발광고속액체크로마토그래피법에 대한 표준물질인 포스파티딜콜린 하이드로퍼옥사이드의 조제

  • Received : 1998.07.04
  • Published : 1998.12.25

Abstract

A simple and rapid method for preparation of standard phosphatidylcholine hydroperoxide (PCOOH) in chemiluminescence-HPLC assay (CL-HPLC) was developed using the rose bengal dependent spectrofluorometric oxidation. The concentration of obtained PCOOH was determined by FOX (ferrous oxidation-xylenol orange) assay and then subjected to a CL-HPLC system.

Keywords

chemiluminescence-HPLC;phosphatidylcholine hydroperoxide;oxidation;ferrous oxidationxylenol orange