Immunohistochemical study on endocrine cells of the thymus of duck(Anas platyrhynchos platyrhyncos, Linne)

청둥오리의 가슴샘 내분비세포에 대한 면역조직화학적 연구

  • Kim, Jeong-mi (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Jae-hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Ku, Sae-kwang (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Hyeung-sik (Department of Biology, College of Natural Science, Kyungsan University)
  • 김정미 (경북대학교 수의과대학) ;
  • 이재현 (경북대학교 수의과대학) ;
  • 구세광 (경북대학교 수의과대학) ;
  • 이형식 (경산대학교 자연과학대학 생물학과)
  • Received : 1998.03.09
  • Published : 1998.06.25

Abstract

Endocrine cells in the thymus of duck(Anas platyrhynchos platyrhyncos, Linne) were studied immunohistochemically from 23 days of incubation to 32 weeks of age. Somatostatin-, growth hormone(GH)-, gastrin/cholecystokinin(Gas/CCK)-, polypeptide YY(PYY)-, S-100 protein(S-100 P)-, dopamine-, serotonin(5-HT)-, and bovine chromogranin (BCG)-immunoreactive cells were detected in the duck thymus by the PAP techniques. These immunoreactive cells were observed in the medulla and in the juxtacortical medulla. No immunoreactivity of calcitonin and bovine pancreatic polypeptide(BPP) antiserum were observed. The argyrophil cells by Grimelius techniques were observed from 23 days of incubation to 32 weeks of age and peaked in 5 weeks of age. In the early develpmental stage, the argyrophil cells were distributed only in the medulla, while these cells were distributed mainly in the medulla and a few cells were distributed in the corticomedullary junction from 3 weeks of age. These immunoreactive cells were generally round, oval and elliptical and occasionally spindle, polygonal and polymorphous with the long cytopslasmic processes in shape. The present study suggests that the intrathymic endocrine cells may associate with the functional maturation of T-lymphocytes on the establishment of immunity. The further study will be needed to elucidate the function of these thymic endocrine cells.