Korean Journal of Veterinary Research (대한수의학회지)
- Volume 38 Issue 2
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- Pages.319-327
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- 1998
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Sequence of the spike gene containing antigenic sites A and D of transmissible gastroenteritis virus isolated in Korea
국내분리 돼지 전염성 위장염 바이러스의 antigenic site A와 D를 포함하는 spike gene의 염기서열 분석
- Kwon, Hyuk-moo (Department of Veterinary Medicine, Kangwon National University) ;
- Pi, Jae-ho (Department of Veterinary Medicine, Kangwon National University) ;
- Seong, Hwan-woo (National Veterinary Research Institute)
- Received : 1998.02.18
- Published : 1998.06.25
Abstract
The nucleotide sequences of spike (S) glycoprotein containing antigenic sites A and D of TGEV isolated in Korea were determined and compared with published sequences for TGEVs. The TGEV 133 and DAE5 strains had 97.40% nucleotide sequence similarity. The overall nucleotide sequence similarity of the 133 and DAE5 strains compared with other TGEV strains was between 96.86% and 99.15%. The similarity of the predicted amino acid sequence of the 133 and DAE5 strains was 94.93%. The TGEV 133 and DAE5 strains had 94.93-98.61% amino acid similarity with published sequences of other TGEV strains. The sequences of amino acid codons in the antigenic sites A and D were identical among all the viruses although there were several nucleotide changes in region containing antigenic sites A and D of Korean TGEV isolates. By phylogenetic analysis of the sequences, two Korean isolates 133 and DAE5 seemed to be derived from different lineages. These studies showed that a distinct difference in genome exists among TGEV field strains isolated in Korea.
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Acknowledgement
Supported by : 한국과학재단