Production and characterization of monoclonal antibodies against porcine transmissible gastroenteritis virus

돼지 전염성 위장염 바이러스에 대한 단크론항체 생산 및 특성

  • Jang, Young-en (College of Veterinary Medicine, Chungbuk National University) ;
  • Cho, Sun-hee (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Byung-han (National Veterinary Research Institute) ;
  • Ahn, Jae-moon (Northern Branch of Chungbuk Office of Agriculture and Livestock) ;
  • Kang, Shien-young (College of Veterinary Medicine, Chungbuk National University)
  • 장영은 (충북대학교 수의과대학) ;
  • 조선희 (충북대학교 수의과대학) ;
  • 김병한 (수의과학연구소) ;
  • 안재문 (충청북도 농축산사업소 북부지소) ;
  • 강신영 (충북대학교 수의과대학)
  • Received : 1998.01.28
  • Published : 1998.06.25

Abstract

Eight monoclonal antibodies(MAbs) against the transmissible gastroenteritis virus (TGEV) were produced and characterized. Four of the MAbs were produced against a reference TGEV, Purdue strain(P115) and the others were produced against the Korean vaccine virus, Pyungtaek strain. Only one MAb(5C8) produced against P115 had neutralizing activity and was found to be E2 protein-specific. The other seven MAbs(4E2, 4G8, 5H6, 1F8, 2C6, 5H5, and 3A6) had specificity of nucleocapsid protein and no neutralizing activity. All MAbs reacted with different strains of TGEV, but none of the MAbs was reactive with porcine enteropathogenic viruses such as rotavirus, epidemic diarrhea virus and enterovirus by fluorescence antibody(FA) test.

Acknowledgement

Supported by : 농촌진흥청