- Volume 31 Issue 1
Rapid Detection of Salmonella spp. by Antibody Immobilization with Gold-protein A Complex
Gold-protein A Complex 항체 고정화법을 이용한 Salmonella spp.의 신속 검출
- Published : 1999.02.28
A piezoelectric (PZ) biosensor system detecting Salmonella spp. was developed. The system consisted of an oscillator, a frequency counter and an antibody-immobilized quartz crystal. An anti-Salmonella antibody was immobilized on one gold. surface of the quartz crystal with protein A. Salmonella detection was made by measuring resonant frequency shift owing to a mass change by specific binding of microbial cells to the gold surface of the PZ crystal. The PZ antibody sensor was operated optimally at 0.1M phosphate buffer, pH 7.2 and
PZ biosensor;antibody immobilization;gold-protein A complex;Salmonella spp.