Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 31 Issue 1
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- Pages.1-6
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- 1999
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- 0367-6293(pISSN)
Rapid Detection of Salmonella spp. by Antibody Immobilization with Gold-protein A Complex
Gold-protein A Complex 항체 고정화법을 이용한 Salmonella spp.의 신속 검출
- Park, In-Seon (Korea Food Research Institute) ;
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Kim, Nam-Soo
(Korea Food Research Institute)
- Published : 1999.02.28
Abstract
A piezoelectric (PZ) biosensor system detecting Salmonella spp. was developed. The system consisted of an oscillator, a frequency counter and an antibody-immobilized quartz crystal. An anti-Salmonella antibody was immobilized on one gold. surface of the quartz crystal with protein A. Salmonella detection was made by measuring resonant frequency shift owing to a mass change by specific binding of microbial cells to the gold surface of the PZ crystal. The PZ antibody sensor was operated optimally at 0.1M phosphate buffer, pH 7.2 and