Determination of Synthetic Food Colours by HPLC with Photodiode Array Detector

HPLC를 이용한 타르색소의 분리정량

  • Yang, Ho-Chul (Food Analysis Division, Chonnam Institute of Health and Environment) ;
  • Heo, Nam-Chil (Food Analysis Division, Chonnam Institute of Health and Environment)
  • 양호철 (전남보건환경연구원 식품분석과) ;
  • 허남칠 (전남보건환경연구원 식품분석과)
  • Published : 1999.02.28


A simple, rapid, efficient method is for extraction of 13 synthetic water-soluble food colours (Tartrazine, Amarnth, Indigo carmine, New coccine, Sunset yellow FCF, Allura red AC, Eosine, Fast Green FCF, Brilliant Blue FCF, Erythrosine, Acid red, phloxine, Rose Bengal) by polyamide resin and for their quantitative by high performance liquid chromatography (HPLC). Colours (coal-tar dyes) were extracted with polyamide resin and then determinated by HPLC. The HPLC conditions using a reverse phase partition type column $(Nova-pak\;C_{18})$, photodiode array (PDA) detector and 1% Ammonium acetate / 60% acetonitrile in water as eluent, were acceptable for various kinds of colorants. By the use of the proposed method, a survey of coal-tar dyes was carried out on 20 samples and that were detected $4.76{\sim}133.47\;ppm$.


Coal-tar dyes;Photodiode array(PDA);HPLC;Polyamide resin