Influence on Lysine Production by Overexpression of the ddh Gene in a Lysine-producing Brevibacterium lactofermentum

Brevibacterium lactofermentum에서 ddh 유전자의 Overexpression이 $_L-Lysine$ 생산에 미치는 영향

  • Park, Sun-Hee (Department of Food & Nutrition, Yeungnam University) ;
  • Kim, Ok-Mi (Department of Food & Nutrition, Yeungnam University) ;
  • Kim, Hyun-Jeong (Department of Food & Nutrition, Yeungnam University) ;
  • Bae, Jun-Tae (Department of Food & Nutrition, Yeungnam University) ;
  • Chang, Jong-Sun (Department of Food & Nutrition, Yeungnam University) ;
  • Lee, Kap-Rang (Department of Food & Nutrition, Yeungnam University)
  • 박선희 (영남대학교 식품영양학과) ;
  • 김옥미 (영남대학교 식품영양학과) ;
  • 김현정 (영남대학교 식품영양학과) ;
  • 배준태 (영남대학교 식품영양학과) ;
  • 장종선 (영남대학교 식품영양학과) ;
  • 이갑랑 (영남대학교 식품영양학과)
  • Published : 1999.02.28

Abstract

The ddh gene encoding meso-DAP-dehydrogenase (DDH) involved in the dehydrogenase pathway is essential for high-level lysine production in Brevibacterium lactofermentum. To investigate its influence on lysine production by overexpression of the ddh gene in a lysine-producing B. lactofermentum, recombinant plasmid pRK1 and pRK31 containing the ddh gene of B. lactofermentum were constructed and they were introduced into B. lactofermentum by electroporation. Multiple copies of pRK1 and pRK31 caused 7-fold and 14-fold increase of DDH activity in B. lactofermentum cell extracts, respectively. As determined in shake flask fermentation, lysine production of B. lactofermentum harboring pRK1 or pRK31 was 22% or 19% higher than that of the control, respectively.

Keywords

Brevibacterium lactofermentum;ddh gene;overexpression;L-lysine production