Bioactive Utility of the Extracts from Rhus verniciflua Stokes (RVS) : Biological Function of the Extracts from RVS

옻나무 추출물의 생리활성 이용에 대한 연구 : 옻나무 추출물의 생물학적 기능

  • Lim, Kye-Taek (Department of Bioactive Substances, Institute of Biotechnology, Chonnam National University) ;
  • Lee, Jeong-Chae (Department of Bioactive Substances, Institute of Biotechnology, Chonnam National University)
  • 임계택 (전남대학교 생물공학연구소 생리활성물질부) ;
  • 이정채 (전남대학교 생물공학연구소 생리활성물질부)
  • Published : 1999.02.28

Abstract

Antioxidative effects of the water or ethanol extracts from Rhus verniciflua Stokes (RVS) were measured by protection against hydroxyl radicals in mouse brain tissue culture. In the water extracts from RVS, cell viabilities were estimated 60.0, 66.0, 72.0, 84.0 and 90.0% at addition of 1, 2, 4, 7 and $10{\mu}L$, respectively, compared with GO (20 mU/mL) alone. The cell viability in the ethanol extracts was similarly with water extracts. In the antitumor effects, the results showed that percentages of the HeLa cell death were approximately 24% for 12 hrs, 57% for 48 hrs at addition of 10%/well ethanol extracts respectively. To know inhibition of tumor growth, in vivo, mice (BALB/c) were inoculated with 0.25 mL CT-26 $(1{\times}10^6\;cells/mL)$ subcutaneously. After the generation of tumor, the results of RVS extracts (ethanol, water) injection showed generally that the tumor size in BALB/c was reduced. For physicochemical characterization of the RVS extracts, purified substances of water or ethanol extracts were analized with SDS-PAGE and ICP spectrometer. In electrophoresis, gel showed 2 bands (210, 230 KDa). The results of ICP verified that RVS extracts contain $Cu^{2+}$ in both samples. Conclusively, this substance might be a laccase which has a biological effective function, as a natural bioactive substance.

Keywords

natural bioactive substance;Rhus verniciflua Stokes (RVS) extracts;hydroxyl radical;antitumor effects