Development of Analysis Method of Gardenia Yellow as Natural Colorants and Content Survey in Commercial Foods by HPLC

HPLC를 이용한 천연착색료인 치자황색소의 분석법 개발 및 시판 식품중 함유량조사

  • Kim, Hee-Yun (Division of Natural Food Additives, Korea Food and Drug Administration) ;
  • Kim, So-Hee (Division of Natural Food Additives, Korea Food and Drug Administration) ;
  • Hong, Ki-Hyoung (Division of Natural Food Additives, Korea Food and Drug Administration) ;
  • Lee, Chul-Won (Division of Natural Food Additives, Korea Food and Drug Administration) ;
  • Kim, Kil-Saeng (Division of Natural Food Additives, Korea Food and Drug Administration) ;
  • Ha, Sang-Chul (Department of Confectionary Decoration, Taegu Future College) ;
  • Jo, Jae-Sun (Department of Food Science and Technology, Kyung Hee University)
  • 김희연 (식품의약품안전청 식품첨가물평가부 천연첨가물과) ;
  • 김소희 (식품의약품안전청 식품첨가물평가부 천연첨가물과) ;
  • 홍기형 (식품의약품안전청 식품첨가물평가부 천연첨가물과) ;
  • 이철원 (식품의약품안전청 식품첨가물평가부 천연첨가물과) ;
  • 김길생 (식품의약품안전청 식품첨가물평가부 천연첨가물과) ;
  • 하상철 (대구미래대학 제과데코레이션과) ;
  • 조재선 (경희대학교 식품공학과)
  • Published : 1999.08.31

Abstract

A simple and practical method for the determination of gardenia yellow in foods was developed. In this method, analysis of gardenia yellow in food products has been carried out by the detection of crocetin and/or geniposide as indicator compounds. As a new analytical method for gardenia yellow, we adopted crocetin, which is produced from colored components of gardenia yellow by alkaline hydrolysis, as an indicator compound. The analysis of gardenia yellow was performed by reverse phase high performance liquid chromatography using a Capcell pak $C_{18}$ column at wave length 240 nm (geniposide) and 435 nm (crocetin). The recovery rates of geniposide and crocetin were found to be 93.4% and 87.8% for Dan Mu Ji, 90.2% and 85.9% for milk, 92.8% and 86.5% for snack, respectively. With this method, the range of crocetin and geniposide contents $({\mu}g/g)$ were as follows: $ND{\sim}1.7$ and $ND{\sim}14.1$ for Dan Mu Ji, $ND{\sim}0.2$ and $ND{\sim}13.6$ for milk, $ND{\sim}1.6$ and $ND{\sim}0.9$ for snack, respectively. The detection limits of crocetin and geniposide were 0.07 ${\mu}g/g$ and 0.05 ${\mu}g/g$, respectively.

Keywords

gardenia yellow;crocetin;geniposide;high performance liquid chromatography