Isolation and Identification of Growth Inhibition Substance on L. monocytogenes from Dystaenia takesimana Kitagawa

섬바디로부터 L. monocytogenes에 대한 생장억제 물질의 분리 및 구조동정

  • Oh, Jin-Ah (Faculty of Biotechnology, Chonbuk National University) ;
  • Shin, Dong-Hwa (Faculty of Biotechnology, Chonbuk National University) ;
  • Baek, Nam-In (Department of Agriculture, Kyunghee University)
  • 오진아 (전북대학교 응용생물공학부(농업과학기술연구소)) ;
  • 신동화 (전북대학교 응용생물공학부(농업과학기술연구소)) ;
  • 백남인 (경희대학교 농학과)
  • Published : 1999.08.31

Abstract

The ethanol extracts and its n-hexane fraction of Dystaenia takesimana Kitagawa exhibited growth inhibition on Listeria monocytogenes ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313. The minimum inhibitory concentration of the ethanol extract and its n-hexane fraction were 50 ppm and below 30 ppm on Listeria monocytogenes respectively. By silica gel column chromatography, the active fraction A8 was obtained from the ethanol extract of Dystaenia takesimana Kitagawa. After three times of column chromatography, the SBD-1 and SBD-2 were separated from the A8 fraction of the ethanol extract of Dystaenia takesimana Kitagawa. Antimicrobial activity of the SBD-l and SBD-2 was lower than that of the A8. And the A8 exhibited growth inhibition on five strains of Listeria monocytogenes at the level of $10{\sim}30$ ppm and the bactericidal effect was confirmed at same the level. The purified antimicrobial active compound was identified as (9z)-heptadeca-l,9-dien-4,6-diyn-3,8-diol, falcarindiol, by EI/MS, $^{1}H-NMR$ and $^{13}C-NMR$.

Keywords

Dystaenia takesimana Kitagawa;L. monocytogenes;antimicrobial activity;polyacetylene;falcarindiol