Isolation of 3,4-Dihydroxybenzoic Acid, Which Exhibits Antimicrobial Activity, from Fruits of Gardenia jasminoides Ellis

치자 열매에서 항미생물 활성을 갖는 3,4-Dihydroxybenzoic Acid의 분리

  • Yim, Cheol-Keun (Department of Food Science and Technology, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Nutrition, University of Tokushima) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • 임철근 (전남대학교 식품공학과) ;
  • 문제학 (일본 덕도대학 의학부 영양학과) ;
  • 박근형 (전남대학교 식품공학과)
  • Published : 1999.10.31


The methanol extract of Gardenia jasminoides Ellis showed antimicrobial activity against bacteria and yeasts. The extract was successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, octadecylsilane column chromatography. The purified active substance was isolated by high performance liquid chromatography. The isolated compound was 3,4-dihydroxybenzoic acid which was determined by mass spectrometer, gas chromatograph-mass spectrometer, $^{1}H-nuclear$ magnetic resonance, $^{13}C-nuclear$ magnetic resonance and two-dimensional nuclear magnetic resonance. The content of 3,4-dihydroxybenzoic acid was $32.7\;{\mu}g/g$ in dried fruits of Gardenia jasminoides Ellis.