Comparative Antimutagenic and Antioxidative Activities of Rice with Different Milling Fractions

도정분획별 쌀의 항돌연변이 및 항산화 활성

  • Chun, Hyang-Sook (Food Chemistry and Biotechnology Division, Korea Food Research Institute) ;
  • You, Jung-Eun (Food Chemistry and Biotechnology Division, Korea Food Research Institute) ;
  • Kim, In-Ho (Food Chemistry and Biotechnology Division, Korea Food Research Institute) ;
  • Cho, Jung-Soon (Department of Food and Nutrition, Myong-Ji University)
  • 전향숙 (한국식품개발연구원 식품생물공학이화학연구본부) ;
  • 유정은 (한국식품개발연구원 식품생물공학이화학연구본부) ;
  • 김인호 (한국식품개발연구원 식품생물공학이화학연구본부) ;
  • 조정순 (명지대학교 식품영양학과)
  • Published : 1999.10.31

Abstract

Antimutagenic and antioxidative activities in the different milling fraction of rice(Oryza sativa L., illpumbyeo) were investigated. Twelve milling fractions including embryo, bran(I, II, III, IV and all) and milled rice(I, II, III, IV and V) and were obtained by abrasive milling. Antimutagenic effects of milling fraction against Trp-P-2-induced mutagenicity were shown as ${\approx}0%$ for embryo fraction, $27{\sim}86%$ for bran fractions and $64{\sim}95%$ for milled rice fractions in salmonela typhimurium reversion assay. Milled rice V, inner fraction with 80.9% milling yields, showed the highest antimutagenic activity among milling fractions Antioxidative activity, measured by peroxide value(POV) of different milling fractions was higher in embryo(28%) and bran fractions ($25{\sim}34%)$ than milled rice fractions($6{\sim}22%)$. In terms of thiobarbituric acid(TBA). embryo. bran and milled rice fractions exhibited 14, $5{\sim}21\;and \;6{\sim}20%$ antioxidative activity, respectively. Antioxidative activity, measured by electron donating ability(EDA), was 45% for embryo fraction. $35{\sim}40%$ for bran fractions and $41{\sim}65%$ for milled rice fractions. Antimutagenic activity if milling fractions was correlated with POV (r=-0.471, p<0.01) and EDA (r = 0.609, p<0.001) but not correlated with TBA. Contents of total phenolic acid and SH were higher in bran and embryo fractions than in milled rice fractions, and were reversely correlated with antimutagenic activity (r=-0.523 and -0.451. respectively, p<0.05).

Keywords

rice;milling fractions;antimutagenicity;antioxidative activity