Isolation and Identification of ${\beta}-Glucuronidase$ producing Clostridium sp. from Fecal Microflora

${\beta}-Glucuronidase$ 생산 인체장내 Clostridium sp.의 분리 . 동정

  • Park, Jong-Hyun (Department of Food and Bioengineering, Kyungwon University) ;
  • Shin, Jee-Young (Department of Food and Bioengineering, Kyungwon University)
  • 박종현 (경원대학교 식품생물공학과) ;
  • 신지영 (경원대학교 식품생물공학과)
  • Published : 1999.10.31

Abstract

For the study of human intestinal environments with the intestinal bacteria producing ${\beta}-glucuronidase\;and \;7{\alpha}-dehydroxylase$, genus Clostridium, known as the producer, were isolated from the fecal microflora. Through screening twice for one person, fecal microflora without major bacterial group seemed to be changed, which indicated the microflora would be changeable by the diet factors. With using Neomycin-Nagler selective medium during the screening, 14 Clostridium spp. were isolated and then the harmful enzyme activities were determined. Isolate-11 among them produced strongly ${\beta}-glucuronidase$ and its activity was 0.021 unit/mg Protein. However, the strain producing $7{\alpha}-dehydroxylase$ was not isolated. The Isolate-11 was tentatively identified as Clostridium scatologenes through cultural and physiological characteristic.

Keywords

${\beta}-glucuronidase$;$7{\alpha}-dehydroxylase$;clostridium sp.;fecal microflora