Macrophage Activation of Chitosan Hydrolysates with Different Molecular Weights in vitro

분자량에 따른 Chitosan의 in vitro 대식세포 활성화

  • Chang, Hyun-Joo (Department of Food and Nutrition, Ewha Womans University) ;
  • Chun, Hyang-Sook (Food Chemistry and Biotechnology Division, Korea Food Research Institute) ;
  • Lee, Su-Rae (Department of Food and Nutrition, Ewha Womans University)
  • 장현주 (이화여자대학교 식품영양학과) ;
  • 전향숙 (한국식품개발연구원 생물공학연구본부) ;
  • 이서래 (이화여자대학교 식품영양학과)
  • Published : 1999.10.31

Abstract

This study was performed to investigate the average molecular weight range of chitosan hydrolysates showing maximum effect in macrophage activation. Nitrite production by continuous macrophage cell line J774A.1 was the highest at $10\;{\mu}g/ml$ concentration of intact chitosan. Hydrogen peroxide production by J774A.1 showed the high value of $894\;{\mu}M/mg$ macrophage protein at $1,000\;{\mu}g/ml$ concentration of chitosan hydrolysate fraction 5 and $1,044\;{\mu}M/mg$ macrophage protein at $100\;{\mu}g/ml$ concentration of the fraction 6. Chitsan hydrolysate fraction 4, fraction 6 and intact chitosan enhanced $IL-1{\alpha}$ production, while the others did not. The production of tumor necrosis factor showed the high value at $1,000\;{\mu}g/ml$ concentration of chitosan hydrolysate fraction 4, $100\;{\mu}g/ml$ concentration of the fraction 5 and fraction 6, and $10\;{\mu}g/ml$ concentration of intact chitosan. In conclusion, fractions 4, 5 and 6 of the chitosan hydrolysatets with average molecular weight of $24,000{\sim}64,000$ calculated by HPLC analysis are the most effective in macrophage activation tested in this study.

Keywords

chitosan hydrolysate;different molecular weight;macrophage activation