Potentiation of Inducible Nitric Oxide Expression by Indomethacin in Carageenin-treated Rat Paw Inflammation

Carrageenin으로 흰쥐 발 염증으로 Indomethacin에 의한 유도성 nitric oxide synthase의 발현증가

  • 원혜영 (서울대학교 약학대학) ;
  • 강건욱 (서울대학교 약학대학) ;
  • 김영미 (국립보건원 특수 질환부 대사질환과) ;
  • 김낙두 (서울대학교 약학대학)
  • Published : 1999.04.01


Present study was aimed to examine whether indomethacin affected the production of NO in the rat paw exudate by carrageenin. Paw edema and nitrite/nitrate levels in the paw exudate were maximal after 4 h and remained elevated up to 10 h, whereas indomethacin (10 mg/kg, po) significantly inhibited the carrageenin-induced paw edema and levels of nitrate in the paw exudate. However, paw edema and nitrite/nitrite levels were increased thereafter for 10 h. Indomethacin also enhanced the expression of iNOS mRNA and protein 4 h after carrageenin infection. Indomethacin inhibited the level of $PGE_2$ in the paw exudate in a time-dependent manner. These results suggest the possibility that indomethacin may potentiate expression of iNOS and subsequently increase nitrite/nitrate level in the late phase of carrageenin-induced rat paw inflammation possibly by suppressing cycloxygenase activity.



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