Purification and Characteristics of Protease Produced by Syncephalastrum racemosum PDA 132-2 from Korean Traditional Meju

메주 유래의 Syncephalastrum racemosum PDA 132-2가 생산하는 Protease의 정제 및 특성

  • 유진영 (한국식품개발연구원, 생물공학연구본부) ;
  • 임성일 (한국식품개발연구원, 생물공학연구본부)
  • Published : 1999.10.01

Abstract

Protease related mold was isolated and selected as a starter culture for commercial production of meju. Isolated microorganism was identified as Syncephalastrum racemosum PDA 132 2. To obtain basic data about protease for production of soybean peptides and application of the strain in meju fermentation, we extrated and purified protease and charateristics of the enzyme were investigated. The optimum condition for the production of enzyme was pH 4.0, 30oC, 5 days. The protease was purified 19.7 folds by gel filtration and ion exchange chromatography and specific activity was 12.4unit/mg. The purified enzyme was 34kDa in size, thiol protease(100% inhibited by PCMB), and was acidic protease(stable between pH 2.0~5.0). Vmax of the enzyme was 2.14 g/min which was lower(1/50) than that of by Asp. wentti and B. subtilis.

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