Increased Production of Recombinant Protein by Escherichia coli Deficient in Acetic Acid Formation

  • Koo, Tae-Young (Department of Genetic Engineering, Sung Kyun Kwan University) ;
  • Park, Tai-Hyun (School of Chemical Engineering, Seoul National University)
  • Published : 1999.12.01


The effect of acetic acid formation deficiency on recombinant E. coli fermentation was investigated using a mutant strain deficient in acetic acid formation. A mutant strain which does not grow under anaerobic conditions was isolated. The acetic acid production in this strain was negligible in aerobic batch fermentation. The cloned-gene expression in the mutant strain was higher than the wild-type strain. Fed-batch fermentations with controlled specific growth rates were carried out in order to compare the cloned-gene expression between the wild-type and the mutant strains. The expression decreased along with the specific growth rate in both strains. The cloned-gene expression in the mutant strain was 60% higher than in the wild-type strain at the same specific growth rate.


  1. Escherichia coli and Salmonell typhimurium v.2 Linkage map of Escherichia coli K-12 Bachmann, B. J.;F. C. Neidhardt(ed.);J. L. Ingraham(ed.);K. B. Low(ed.);B. Magasanik;M. Schaechter(ed.)
  2. Appl. Microbiol. Biotechnol. v.23 Continuous production of human leukocyte interferon with Escherichia coli and continuous cell lysis in a two stage chemostat. Brown, S. W.;H. Meyer;A. Fiechter
  3. J. Microbiol. Biotechnol. v.7 Periplasmic expression of a recombinant antibody (MabB9) in Escherichia coli. Chang, H.-C.;J. W. Kwak
  4. Biotechnol. Bioeng. v.38 Effect of alteration of the acetic acid synthesis pathway on the fermentation pattern of Escherichia coli Diaz-Ricci, J. C.;L. Regan;J. E. Bailey
  5. Adv. Biochem. Eng. v.23 Regulatioon of glucose metabolism in bacterial systems Doelle, H. W.;K. N. Ewing;N. W. Hollywood
  6. Chem. Eng. Comm. v.45 Use of novel air separation system in a fed-batch fermentative culture of Escherichia coli Fass, R.;T. R. Clem;J. Shiloach
  7. Cold Spring Harbor Sym. Quant. Biol. v.45 Identification of a sex-factor-affinity site in E. coli as γδ Guyer, M. S.;R. R. Reed;J. A. Steitz;K. B. Low
  8. Biotechnol. Bioeng. v.41 Relieving effects of glycine and methionine from acetic acid inhibition in Escherichia coli fermentation Han, K.;J. Hong;H. C. Lim
  9. Biotechnol. Bioeng. v.39 Acetic acid formation in Escherichia coli fermentation Han, K.;H. C. Lim;J. Hong
  10. Curr Topics Cell Regul. v.28 The central metabolic pathways of Escherichia coli: Relationship biomass and excretion of acetate Holmes, W. H.
  11. Kor. J. Biotechnol. Bioeng. v.9 Hollow fiber dialysis culture of E. coli Kim, I. H.;T. H. Yoon
  12. J. Microbiol. Biotechnol. v.7 Design and expresion of high nutritional peptide (HEAAE) in E. coli Kim, I. H.;T. H. Yoon
  13. J. Microbiol. Biotechnol. v.8 Overexpression of Escherichia coli thiol peroxidase in the periplasmic space Kim, S.-J.;M.-K. Cha;I.-H. Kim;H.-K. Kim
  14. J. Microbiol. Biotechnol. v.8 Expression of the EPO-like domains of human thrombopoietin in Escherchia coli Koh, Y. W.;T. Y. Koo;S. M. Ju;C. H. Kwon;J. Y. Chung;M. H. Park;J. M. Yang;s. K. Park
  15. Kor. J. Biotechnol. Bioeng. v.10 Effect of acetic acid formation and specific growth rate on productivity of recombinant Escherichia coli fed-batch fermentation Koo, T. Y.;T. H. Park
  16. J. Microbiol. Biotechnol. v.8 Expression of an antimicrobial peptide maganin by a promoter inversion system Lee, J.-H.;S.-S. Hong;S.-C. Kim
  17. Biotechnol. Bioeng. v.35 Simple constrained optimization view of acetate overflow in E. coli Majewski, R. A.;M. M. Domach
  18. Nucleic Acid Research v.16 Search for the optimal sequence of the ribosome binding site by random oligonucleotide-directed mutagenesis Min, K. T.;M. H. Kim;D. S. Lee
  19. J. Ferment. Technol. v.50 Kinetic studies on submerged acetic acid fermentation (IV): Product inhibition and transient adaptation of cells to the product Mori, A.;H. Yoshikawa;G. Terui
  20. J. Biotechnol. v.12 Continuous production of acetic acid by electrodialysis bioprocess with computerized control of fed-batch culture Nomura, Y.;M. Iwahara;M. Hongo
  21. J. Microbiol. Bioitechnol. v.7 Overexpression of Escherichia coli-D-xylose isomerase using λP₁promoter Park, H.-D.;G.-J. Joo;I.-K. Rhee
  22. Nature v.174 Iodometric assay of penicillinase Perret, C. J.
  23. J. Biotechnol. v.2 Mass culture of Escherichia coli: Medium complex media Reiling, H. E.;H. Laurila;A. Fiechter
  24. J. Microbiol. Biotechnol. v.8 Polyglutamine residues from Machado-Joseph disease gene enhance formation of aggregates of GST-polyglutamine fusion protein in E. coli Rhim, H.;K.-S. Bok;M.-J. Chang;I-K. Kim;S. S. Park;S. Kang
  25. J. Bacteriol. v.95 Rapid fixed-time assay for penicillinase Sargent, M. G.
  26. Antimicrob. Agents and Chemother v.13 Iodometric assay method for β-lactamase with various β-lactam antibiotics as substrate Sawai, T.;I. Takahashi;S. Yamagishi
  27. J. Ferment. Technol. v.66 Fed-batch cultures of recombinant Escherichia coli with inhibitory substance concentration monitoring Shimizu, N.;S. Fujuzono;K. Fujimory;N. Nishimura;Y. Odawara
  28. Biotechnol. Bioeng. v.43 Fed-batch operation of recombinant Escherichia coli containing trp promoter with controlled specific growth rate Yoon, S. K;W. K. Kang;T. H. Park
  29. J. Ferment. Bioeng. v.81 Regulation of trp promoter for production of bovine somatotropin in recombinant Escherichia coli fed-batch fermentation Yoon, S. K.;W. K. Kang;T. H. Park
  30. Biotechnol. Lett. v.16 Optimization of recombinant Escherichia coli fed-batch fermentation for bovine somatotropin Yoon, S. K.;S. H. Kwon;M. G. Park;W. K. Kang;T. H. Park