Determination of Glucose Using Enzyme Immobilized Membrane

효소 고정화 막을 이용한 Glucose의 정량

  • Kim, Im Ok (Department of Chemistry, Gyeongsang National University) ;
  • Kwak, Kyeong Do (Department of Chemistry, Gyeongsang National University) ;
  • Ha, Youn Shick (Department of Chemical engineering, Inha University) ;
  • Kwon, Hyo Shik (Department of Chemical Edu., Chungbuk National University) ;
  • Seo, Moo Lyong (Department of Chemistry, Gyeongsang National University)
  • 김임옥 (경상대학교 자연과학대학 화학과) ;
  • 곽경도 (경상대학교 자연과학대학 화학과) ;
  • 하윤식 (인하대학교 공과대학 화학공학과) ;
  • 권효식 (충북대학교 사범대학 화학교육과) ;
  • 서무룡 (경상대학교 자연과학대학 화학과)
  • Published : 19990600

Abstract

Enzyme electrodes for amperometric measurement of glucose were prepared by immobilization of glucose oxidase on an Immobilon-AV Affinity membrane and attachement to a Pt electrodes. The electrochemical oxidation of Hz02 was monitored at +0.8V vs. Ag/AgCl. Response was linear from 0.2 mM to 20mM. The detection limit was 10m3 mM. Response time, the optimum pH and life time of enzyme immobilized membrane was 12 seconds, pH 5.5(CH3COONaJCH3COOH) and about 27 days, respectively. When the enzyme electrode was applied for the determinaion of glucose with amperometric method, other physiolosical materials have not interfered. Also, we compared the result with that from AOAC(Association of Offical Analytical Chemists) method, measuring the glucose in sweet potato. The relative error was 0.1%.

Keywords

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