Determination of Aldehydes in Tap Water by Reverse Phase Liquid Chromatography

역상 액체 크로마토그래피에 의한 수도수 중 알데하이드류의 정량

  • 최용욱 (전주대학교 화학.신소재학과) ;
  • 최윤정 (한국건설기술연구원 수자원환경부)
  • Published : 19990800

Abstract

The optimum analytical method of aldehydes, ozone by-products, was established by reverse phase liquid chromatography. Six aldehydes including formaldehyde, acetaldehyde, acrolein, propionaldehyde, butylaldehyde and benzaldehyde, and one ketone including acetone were selected as aldehyde test samples through preliminary experiments. Such analytical conditions as the pH of citrate buffer solution, reaction temperature, reaction time, and concentration of DNPH, the component and composition of desorption solvent were optimized. As the result, pH 3.0 of citrate buffer solution, 40$^{\circ}C$ of reaction temperature, 15 minutes of reaction time, and 0.012% of DNPH concentration were chosen as optimum conditions. Aldehydes-DNPH derivatives in water were concentrated on $C_18$ Sep-Pak cartridge and followed by elution of their derivatives fraction with THF/ACN(70/30) mixture, and showed recoveries of the range from 87 to 107%. Separation condition on Nova-Pak $C_18$ column with low pressure gradient elution from ACN/MeOH/water(30/10/60) of an initial condition to 80% ACN of a final condition was found to give a good resolution within 20 minutes of run time. 86% to 103% of recovery for aldehydes using this method was similar to that for aldehyde using EPA Method 554 which is ranged from 84% to 103%.

Keywords

References

  1. Analyst v.119 Maeda, T.;Hu, X.;Itou, S.;Kitano, M.;Tadenaka, N.;Bandow, H.;Munemori, M.
  2. Anal. Chem. v.53 Midsch, R. R.;Anton, D. W.;Fanning, L. Z.;Hollowell, C. D.;Revzan, K.;Glanville, J.
  3. Anal. Chem. v.54 Kennedy, E. R.;Hill, R. H.
  4. J. Chroamtogr. v.455 Ricking, M. K. L.;Cooke, M.;Dawahara, F. K.;Lonhbottom, J. E.
  5. Environ. Sci. Technol. v.27 Lee, Y. N.;Zhou, Z.
  6. Anal. Chem. v.57 Takami, K.;Kuwata, K.;Sugimae, A.;Nakamoto, M.
  7. 연세대학교 박사학위논문 임영옥
  8. 한국과학기술 연구원 연구보고서 박종세(외 9명)
  9. Anal. Chem. v.64 Dietrich A. M.;Ledder, T. D.;Gallagher, D. L.;Grabeal, M. N.;Hoehn, R. C.
  10. 건설 기술 정보 이현동
  11. Environ. Sci. Technol. v.24 no.10 Kieber, R. J.;Mopper. K.
  12. Environ. Sci. Technol. v.23 no.7 Glaze, W. H.;Koga, M.;Cancilla, D.
  13. 水道協力雜誌 v.64 no.4 Ozawa, S.;Aizawa, T.;Nakazima, H.;Shinhei, H.
  14. 한국과학기술 연구원 연구보고서 박종세(외 9명)
  15. J. Chromatogr. v.329 Ogawa, I.;Fritz, J. S.
  16. J. Am. Water Work Assoc. v.86 Weinverg, H. S.;Glaze, W. H.;Krasner, S. W.;Sclimenti, M. J.
  17. Bunseki Kagaku v.47 Nishikawa H.;Magasawa, H.;Sakai T.
  18. J. Chromatogr. Sci. v.17 Kuwata, K.;Uebori, M.;Yamasaki, Y.
  19. 연세대학교 석사학위논문 김준성
  20. J. Am. Water Work Assoc. v.81 no.8 Krasner, S. W.;McGuire, M. J.;Jacangelo, J. G.;Patania, N. L.;Reagan, K. M;Aieta, E. M.
  21. 한국과학기술원 연구보고서 박종세;박종자(외 8명)
  22. Anal. Chem. v.55 Georghiou, P. E.;Harlick, L.;Winsor, L.;Snow, D.
  23. Anal. Chem. v.47 Slawinska D.;Slawinski J.
  24. Environ. Sci. Technol. v.16 no.10 Hileman, B.
  25. USEPA Method 554
  26. 한국과학기술원 연구 보고서 박종세;박종자(외 7명)