Changes of Silk Protein Compositions by Solubility Condition

용해조건에 따른 견 단백질의 조성 변화

  • Yeo, Joo-Hong (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technology) ;
  • Lee, Kwang-Gill (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technology) ;
  • Lee, Yong-Woo (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technology) ;
  • Nam, Jin (Department of Natural Fiber Science, Seoul National University) ;
  • Kim, Sun-Yeou (Department of Sericulture and Entomology, National Institute of Agriculture Science and Technology)
  • 여주홍 (농업과학기술원 잠사곤충부) ;
  • 이광길 (농업과학기술원 잠사곤충부) ;
  • 이용우 (농업과학기술원 잠사곤충부) ;
  • 남진 (서울대학교 천연섬유학과) ;
  • 김선여 (농업과학기술원 잠사곤충부)
  • Received : 1999.02.04
  • Published : 1999.08.25

Abstract

Changes of silk protein compositions of average molecular weight (Mw) and free amino acid composition to different solubility conditions were studied by SDS-polyacrylamide electropholesis, gel permeation chromatography (GPC), and free amino acid analysis method. We can not detected average molecular weight distribution of different hydrochloric acid (HCl) conditions as SDS-polyacrylamide method, but as using GPC method, molecular weight distribution of 2N-HCl, 1N-HCl and 0.5N-HCl (3 hrs at $110^{\circ}C$ treated) are confirmed Mw 800, 1,500 and 3,700, respectively. The average molecular weight of calcium chroride and calcium chloride-enzyme treated samples are shown Mw 46,800 and 12,500, respectively. The degree of hydrolysis and the composition of the free amino acid in the fibroin hydrolysates effected significantly composition of free amino acids of the fibroin powder. The increase of the degree of hydrolysis and ratio of free amino acids and oligopeptides were found to be directly related to the concentration of hydrochloric acid and treatment of enzyme, resulting in the increase of water solubility.

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