Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis

모세관 전기영동법을 이용한 1,2,4-트리메틸벤젠 대사체의 분석

  • Received : 1999.03.19
  • Published : 1999.08.25

Abstract

The metabolites of 1,2,4-trimethylbenzene (TMB) were synthesized and determined by capillary electrophoresis (CE). The optimum conditions of CE for the separation and determination of 3,4-, 2,4-, 2,5-dimethylbenzoic acid and 3,4-, 2,4-, 2,5-dimethylhippuric acid from the rat urine were as following: the fused silica capillary($75{\mu}m$ i.d. ${\times}$ 36 cm length, 29 cm to detector) was used and kept at $15^{\circ}C$. The applied voltage was 10㎸ and compounds were detected at UV 210 mnm and 254 nm. The running electrolyte was 0.1 M phosphate buffer (pH 7) containing 15 mM of ${\beta}-CD$ and 3% of 2-propanol. The relative amount of the metabolite of 1,2,4-TMB in the rat urine was 56.7% of 3,4-isomer, 30.5% of 2,4-isomer and 12.8% of 2,5-isomer. This method can be applied to the analysis of TMB-metabolites in human urine.

Keywords

capillary electrophoresis;trimethylbenzene;dimethylbenzoic acid;dimethylhippuric acid;metabolites

Acknowledgement

Supported by : 충남대학교 약학대학

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