Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis

모세관 전기영동법을 이용한 1,2,4-트리메틸벤젠 대사체의 분석

  • Received : 1999.03.19
  • Published : 1999.08.25


The metabolites of 1,2,4-trimethylbenzene (TMB) were synthesized and determined by capillary electrophoresis (CE). The optimum conditions of CE for the separation and determination of 3,4-, 2,4-, 2,5-dimethylbenzoic acid and 3,4-, 2,4-, 2,5-dimethylhippuric acid from the rat urine were as following: the fused silica capillary($75{\mu}m$ i.d. ${\times}$ 36 cm length, 29 cm to detector) was used and kept at $15^{\circ}C$. The applied voltage was 10㎸ and compounds were detected at UV 210 mnm and 254 nm. The running electrolyte was 0.1 M phosphate buffer (pH 7) containing 15 mM of ${\beta}-CD$ and 3% of 2-propanol. The relative amount of the metabolite of 1,2,4-TMB in the rat urine was 56.7% of 3,4-isomer, 30.5% of 2,4-isomer and 12.8% of 2,5-isomer. This method can be applied to the analysis of TMB-metabolites in human urine.


capillary electrophoresis;trimethylbenzene;dimethylbenzoic acid;dimethylhippuric acid;metabolites


Supported by : 충남대학교 약학대학


  1. Toxicol. Lett. v.77 P. Kostrewski;A. Wiaderna-Brycht
  2. The Aldrich Library of ${^13}C$ and ¹H FT NMR Spectra v.12 C. J. Pouchert;J. Behnke
  3. Int. J. Occup. Med. Environ. Health v.9 Z. Korsak;K. Rydzynski
  4. Int. J. Occup. Med. Environ. Health v.64 G. Tribig;K. H. Schaller;D. Weltle
  5. Pharmacol. Toxicol. v.67 K. Zahlsen;A. M. Nilsen;I. Eide;O. G. Nilsen
  6. Arch. Toxicol. v.45 J. Cerf;M. Potvin;S. Laham
  7. Int. J. Occup. Med. Environ. Health v.64 M. Ichiba;H. Hama;S. Yukitake;M. Kubota;S. Kawasaki;K. Tomokuni
  8. Xenobiotica. v.19 J. Z. Huo;S. Aldous;K. Campbell;N. Davies
  9. Organic Syntheses v.2 A. W. Ingersoll;S. H. Babocock;A. H. Blatt(Ed.)
  10. Int. J. Occup. Med. Environ. Health v.69 B. Stahlbom;J. Jarnberg;P. Soderkvist;D. Lindmark
  11. Int. J. Occup. Med. Environ. Health v.65 Y. Fukaya;I. Saito;T. Matsumoto;Y. Takeuchi;S. Tokudome
  12. Toxicol. Appl. Pharmacol. v.140 J. Jarnberg;G. Johanson;A. Lof
  13. Int. J. Occup. Med. Environ. Health v.8 Z. Korsak;R. Swiercz;K. Rydzynski
  14. Int. J. Occup. Med. Environ. Health v.9 J. A. Indulksi;H. Sinczuk-Walczak;M. Szymczak;W. Wesolowski
  15. J. Chromatogr. v.577 S. Busini;P. Bavazzano;E. Occhiato
  16. Yakhak Hoeji. v.41 J. M. Lim;J. Y. Jang;Y. M. Lee;J. S. Kang
  17. Toxicol. Appl. Pharmacol. v.11 J. Jarnberg;G. Johanson
  18. J. Chromatogr. v.337 P. Pfffli;H. H rk nen;H. Savolainen