Fundamental Investigation of Non-invasive Determination of Alcohol in Blood by Near Infrared Spectrophotometry

근적외선 분광분석법을 이용한 음주측정기술 개발에 관한 연구

  • 장수현 (동덕여자대학교 약학과) ;
  • 조창희 (동덕여자대학교 약학과) ;
  • 우영아 (동덕여자대학교 약학과) ;
  • 김효진 (동덕여자대학교 약학과) ;
  • 김영만 (한국과학기술연구원 특성분석센터) ;
  • 이강붕 (한국과학기술연구원 특성분석센터) ;
  • 김영운 (국립과학수사연구소) ;
  • 박성우 (국립과학수사연구소)
  • Received : 1999.04.15
  • Published : 1999.10.25

Abstract

Near infrared spectrophotometry(NIR) was developed as a non-invasive determination of blood alcohol. The first pure alcohol/water samples were prepared with ethanol concentration from 0.01 to 0.1%(w/w). Analysis of the second-derivative data was accomplished with multilinear regression(MLR). The standard error of calibration(SEC) of ethanol in ethanol/water solutions was approximately 0.0039%. The calibration models were established from the blood alcohol spectra by MLR and PLSR analysis. The best calibration was built with the second-derivative spectra of 2266 and 2326 nm by MLR. Second-derivative spectra in the spectral ranges of 1100~1340, 1500~1796 and 2064~2300 nm with four PLSR factors provided the standard error of prediction(SEP) of 0.030%(w/w). These results indicate that NIR may be applied for a fast non-invasive determination of alcohol in the blood.

Keywords

near infrared spectrophotomeuy(NIR);non-invasive determination;alcohol;multiple linear regression(MLR);panial least squares regression(PLSR)

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