Identification of Korean Native Goat Meat using DNA Analysis

DNA분석기법을 이용한 한국재래산양육의 판별

  • Sang, B.C. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Lee, S.H. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Ryoo, S.H. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Seo, K.W. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Han, S.W. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Kim, S.K. (Dept. of Animal Science, College of Woosong Information)
  • 상병찬 (충남대학교 농과대학 동물자원학부) ;
  • 이상훈 (충남대학교 농과대학 동물자원학부) ;
  • 류승희 (충남대학교 농과대학 동물자원학부) ;
  • 서길웅 (충남대학교 농과대학 동물자원학부) ;
  • 한성욱 (충남대학교 농과대학 동물자원학부) ;
  • 김선균 (우송정보대학 동물과학과)
  • Published : 1999.12.30

Abstract

This study was carried out to analyze the genetic polymorphisms of genomic DNA of blood and meat for conservation of the genetic resources and genetic improvement of Korean Native goat. The genetic identification between Korean Native goat and imported goat was examined using RAPD(random amplified polymorphisms DNAs) analysis with 30 Korean Native goat, 10 hybrid, 10 imported goat. 10 Korean native goat meat and 10 imported goat meat. The results obtained from this study were summarized as follows: 1. Genomic DNA from Korean native goat, hybrid and imported goat could be obtained above about 23kb size using 0.5% agarose gel electrophoresis and the ratio of optical density at 260nm to that at 280nm was between 1.7 and 2.0 using UV spectrophtometer instrument. 2. In the results of the gene identification between Korean Native goat and hybrid, and imported goat using RAPD methods with random primer of 110 kinds, only Korean native goat showed a specific band at about 369bp using a random primer OPO-19 (5'-CAA ACG TCG G-3'), but imported goat and hybrid not showed. 3. Also, in the results of the gene identification between Korean Native goat meat and imported goat meat using RAPD methods with random primer, Korean native goat only showed a specific band at about 369bp using a random primer No. 19(5'-CAA ACG TCG G-3'), but imported goat not showed.

Keywords

Acknowledgement

Supported by : 한국과학재단