Analysis of Genetic Polymorphisms and Similarity Using Random Amplified Polymorphic DNAs in Cattle

RAPD기법을 이용한 축우의 유전적 다형성과 유사도 분석

  • Lee, S.H. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Seo, K.W. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
  • Kwon, I. (Dept. of Food Science and Technology, College of Agriculture, Chungnam National University) ;
  • Sung, C.K. (Dept. of Food Science and Technology, College of Agriculture, Chungnam National University) ;
  • Kim, S.K. (Dept. of Animal Science, College of Woosong Information) ;
  • Sang, B.C. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University)
  • 이상훈 (충남대학교 농과대학 동물자원학부) ;
  • 서길웅 (충남대학교 농과대학 동물자원학부) ;
  • 권일 (충남대학교 농과대학 식품공학과) ;
  • 성창근 (충남대학교 농과대학 식품공학과) ;
  • 김선균 (우송정보대학 동물과학과) ;
  • 상병찬 (충남대학교 농과대학 동물자원학부)
  • Published : 1999.12.30

Abstract

This study was carried out to investigate the difference and genetic similarity at the level of molecular genetics. Genomic DNA was extracted from blood of Holstein, Korean cattle, Charolais, and hybrid between Korean cattle and charolais and RAPD(random amplified polymorphic DNAs) was analyzed by PCR(polymerase chain reaction). After genetic similarity value from different breeds are analyzed, genetic similarity was estimated by UPGMA(unweighted pair-group method using average). The results obtained from this study can be summarized as follows: 1. When genomic DNA which was extracted from different breeds was subjected to electrophoresis on 1.5% agarose gel, bigger than 12.2kb was appeared. Ratio by absorbance of $A_{260}/A_{280}$ was 1.75~2.10, indicating that genomic DNA was quite pure for RAPD analysis. 2. Different band patterns by RAPD were appeared according to the breeds in cattle. The best primer used to distinguish Holstein from other breeds was 5'-GAC CGC TTG T-3'. 3. A 340bp fragment was amplified in $33.0^{\circ}C$ of annealing temperature for the Holstein and Charolais breeds, but any amplification was not occurred in this annealing temperature for Korean cattle and hybrid. In addition, a 340bp fragment was amplified in $37.5^{\circ}C$ of annealing temperature for the Holstein and Korean cattle, but any amplification was not occurred in this annealing temperature for Charolais and hybrid. For the reaction of PCR. $37.5^{\circ}C$ and $33.0^{\circ}C$ of annealing temperature was shown to be best for genetic marker identification from Holstein, Charolais, and hybrid between Korean cattle and Charolais. 4. When genetic similarity from different breeds are analyzed at the both temperature of $33.0^{\circ}C$ and $37.5^{\circ}C$, the genetic similarity value of Holstein and Korean cattle, Holstein and Charolais, Korean cattle and Charolais, and Korean cattle and hybrid were 0.666~0.777, 0.615~0.666, 0.400~0.461 and 0.857~0.888, respectively. 5. It could be concluded that different breeds are capable of distinguishing by RAPD used random primer 5'-GAC CGC TTG T-3', genetic similarity from different breeds was appeared the higher genetic similarity value of Korean cattle and Charolais than that of Holstein between Korean cattle and Charolais by UPGMA.

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