DOI QR코드

DOI QR Code

Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen - Review-

  • White, B.A. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Cann, I.K.O. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Kocherginskaya, S.A. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Aminov, R.I. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Thill, L.A. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Mackie, R.I. (Department of Animal Sciences, University of Illinois at Urbana-Champaign) ;
  • Onodera, R. (Miyazaki University, Animal Science Division, Animal Nutrition and Biochemistry)
  • Published : 1999.01.01

Abstract

If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and reed panicles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing pupulation dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.

Keywords

Rumen Microbes;DNA;RNA;Ecology;Molecular Analysis;DGGE;Review

Cited by

  1. Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model vol.6, pp.9, 2004, https://doi.org/10.1111/j.1462-2920.2004.00575.x
  2. Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing vol.7, pp.4, 2005, https://doi.org/10.1111/j.1462-2920.2005.00721.x
  3. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR vol.75, pp.1, 2007, https://doi.org/10.1007/s00253-006-0802-y
  4. Recent developments in nucleic acid based techniques for use in rumen manipulation vol.38, pp.spe, 2009, https://doi.org/10.1590/S1516-35982009001300034
  5. Phylogenetic analysis of 16S rRNA gene sequences reveals rumen bacterial diversity in Yaks (Bos grunniens) vol.37, pp.1, 2010, https://doi.org/10.1007/s11033-009-9794-x
  6. — Invited Review — Metagenomic investigation of gastrointestinal microbiome in cattle vol.30, pp.11, 2017, https://doi.org/10.5713/ajas.17.0544
  7. A metagenomic study of the rumen virome in domestic caprids pp.1432-8798, 2018, https://doi.org/10.1007/s00705-018-4022-4