야생등줄쥐(Apodemus agrarius) 후각망울의 neuropeptide Y 면역반응세포의 분포

Distribution of the neuropeptide Y immunoreactive neurons in the olfactory bulb of striped field mouse(Apodemus agrarius)

  • 정영길 (건양대학교 의과대학 해부학교실) ;
  • 김길수 (아산생명과학연구소 실험동물연구실) ;
  • 정주영 (충남대학교 수의과대학) ;
  • 이남섭 (건양대학교 의과대학 해부학교실) ;
  • 이경렬 (충남대학교 수의과대학) ;
  • 김무강 (충남대학교 수의과대학)
  • Jeong, Young-gil (Department of Anatomy, College of Medicine, Konyang University) ;
  • Kim, Kil-soo (Department of Laboratory Animal Research, ASAN Institute for Life Sciences) ;
  • Jung, Ju-young (College of Vetrinary Medicine, Chungnam National University) ;
  • Lee, Nam-seob (Department of Anatomy, College of Medicine, Konyang University) ;
  • Lee, Kyeng-youl (College of Vetrinary Medicine, Chungnam National University) ;
  • Kim, Moo-kang (College of Vetrinary Medicine, Chungnam National University)
  • 투고 : 1999.01.30
  • 발행 : 1999.06.22

초록

This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{\mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.

과제정보

연구 과제 주관 기관 : 한국과학재단