Effect of transmural stimulation to motility on isolated gastric smooth muscle

토끼 적출 위 평활근의 운동성에 대한 transmural stimulation의 효과

  • Kim, Joo-heon (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Shim, Cheol-soo (Eastern Branch, Gyeongnam Livestock Promotion Institute) ;
  • Hong, Yong-geun (Department of Biochemistry, College of Natural Sciences, Gyeongsang National University)
  • 김주헌 (경상대학교 수의과대학, 동물의학연구소) ;
  • 심철수 (경남도축산진흥연구소 동부지소) ;
  • 홍용근 (경상대학교 자연과학대학 생화학과)
  • Received : 1999.03.09
  • Published : 1999.06.22

Abstract

To elucidate the action of the cholinergic and adrenergic nerve on isolated gastric fundus smooth muscle of rabbit, the effects of electrical transmural stimulation were investigated in the presence of atropine, cholinergic receptor blocker; phentolamine, nonselective ${\alpha}$-adrenergic receptor blocker; propranolol, nonselective ${\beta}$-adrenergic receptor blocker and L-arginine from the isometric contraction of physiological recording system. 1. The contractile response induced by electrical transmural stimulation was increased as the frequency(1~32Hz)-dependent manner on the isolated gastric fundus smooth muscle. 2. The contractile response induced by electrical transmural stimulation was markedly inhibited by the pretreatment of atropine($1{\mu}M$). 3. The contractile response induecd by electrical transmutal stimulation was inhibited by the pretreatment of phentolamine($1{\mu}M$). 4. The relaxative response induced by electrical transmural stimulation on presence of atropine ($1{\mu}M$) was inhibited by the pretreatment of propranolol($1{\mu}M$). 5. The relaxative responses on precontraction induced by histamine($10{\mu}M$) with guanethidine ($50{\mu}M$) and atropine($1{\mu}M$) by electrical transmural stimulation were increased by L-arginine (1mM). These findings suggest that it was the excitatory action of cholinergic and ${\alpha}$-adrenergic nerve, and the inhibitory action of ${\beta}$-adrenergic nerve and nonadrenergic noncholinergic nerve on the isolated gastric fundus smooth muscle of rabbit.

Acknowledgement

Supported by : 경상대학교